Characterization of an endogenous morphine like factor (Enkephalin) in mammalian brain

G. W. Pasternak, R. Simantov, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Mammalian brain extracts contain a substance, ''enkephalin'', which competes for opiate receptor binding in a highly selective fashion. This material behaves in opiate receptor assays as an opiate agonist, with its effects enhanced by manganese and decreased by sodium. The regional distribution of enkephalin parallels that of the opiate receptor itself. Enkephalin activity is degraded by carboxypeptidases A and B and leucinaminopeptidase as well as by chymotrypsin, but is resistant to trypsin and neuraminidase. Thus enkephalin appears to be a peptide. Gel filtration experiments indicate a molecular weight of about 1000.

Original languageEnglish (US)
Pages (from-to)504-513
Number of pages10
JournalMolecular Pharmacology
Volume12
Issue number3
StatePublished - 1976

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Enkephalins
Morphine
Opioid Receptors
Brain
Opiate Alkaloids
Carboxypeptidase B
Carboxypeptidases A
Chymotrypsin
Neuraminidase
Manganese
Trypsin
Gel Chromatography
Molecular Weight
Sodium
Peptides

ASJC Scopus subject areas

  • Pharmacology

Cite this

Characterization of an endogenous morphine like factor (Enkephalin) in mammalian brain. / Pasternak, G. W.; Simantov, R.; Snyder, Solomon H.

In: Molecular Pharmacology, Vol. 12, No. 3, 1976, p. 504-513.

Research output: Contribution to journalArticle

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