TY - JOUR
T1 - Characterization of adipocyte differentiation from human mesenchymal stem cells in bone marrow
AU - Qian, Shu Wen
AU - Li, Xi
AU - Zhang, You You
AU - Huang, Hai Yan
AU - Liu, Yuan
AU - Sun, Xia
AU - Tang, Qi Qun
PY - 2010
Y1 - 2010
N2 - Background. Adipocyte hyperplasia is associated with obesity and arises due to adipogenic differentiation of resident multipotent stem cells in the vascular stroma of adipose tissue and remote stem cells of other organs. The mechanistic characterization of adipocyte differentiation has been researched in murine pre-adipocyte models (i.e. 3T3-L1 and 3T3-F442A), revealing that growth-arrest pre-adipocytes undergo mitotic clonal expansion and that regulation of the differentiation process relies on the sequential expression of three key transcription factors (C/EBP, C/EBP and PPAR). However, the mechanisms underlying adipocyte differentiation from multipotent stem cells, particularly human mesenchymal stem cells (hBMSCs), remain poorly understood. This study investigated cell cycle regulation and the roles of C/EBP, C/EBP and PPAR during adipocyte differentiation from hBMSCs. Results. Utilising a BrdU incorporation assay and manual cell counting it was demonstrated that induction of adipocyte differentiation in culture resulted in 3T3-L1 pre-adipocytes but not hBMSCs undergoing mitotic clonal expansion. Knock-down and over-expression assays revealed that C/EBP, C/EBP and PPAR were required for adipocyte differentiation from hBMSCs. C/EBP and C/EBP individually induced adipocyte differentiation in the presence of inducers; PPAR alone initiated adipocyte differentiation but the cells failed to differentiate fully. Therefore, the roles of these transcription factors during human adipocyte differentiation are different from their respective roles in mouse. Conclusions. The characteristics of hBMSCs during adipogenic differentiation are different from those of murine cells. These findings could be important in elucidating the mechanisms underlying human obesity further.
AB - Background. Adipocyte hyperplasia is associated with obesity and arises due to adipogenic differentiation of resident multipotent stem cells in the vascular stroma of adipose tissue and remote stem cells of other organs. The mechanistic characterization of adipocyte differentiation has been researched in murine pre-adipocyte models (i.e. 3T3-L1 and 3T3-F442A), revealing that growth-arrest pre-adipocytes undergo mitotic clonal expansion and that regulation of the differentiation process relies on the sequential expression of three key transcription factors (C/EBP, C/EBP and PPAR). However, the mechanisms underlying adipocyte differentiation from multipotent stem cells, particularly human mesenchymal stem cells (hBMSCs), remain poorly understood. This study investigated cell cycle regulation and the roles of C/EBP, C/EBP and PPAR during adipocyte differentiation from hBMSCs. Results. Utilising a BrdU incorporation assay and manual cell counting it was demonstrated that induction of adipocyte differentiation in culture resulted in 3T3-L1 pre-adipocytes but not hBMSCs undergoing mitotic clonal expansion. Knock-down and over-expression assays revealed that C/EBP, C/EBP and PPAR were required for adipocyte differentiation from hBMSCs. C/EBP and C/EBP individually induced adipocyte differentiation in the presence of inducers; PPAR alone initiated adipocyte differentiation but the cells failed to differentiate fully. Therefore, the roles of these transcription factors during human adipocyte differentiation are different from their respective roles in mouse. Conclusions. The characteristics of hBMSCs during adipogenic differentiation are different from those of murine cells. These findings could be important in elucidating the mechanisms underlying human obesity further.
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U2 - 10.1186/1471-213X-10-47
DO - 10.1186/1471-213X-10-47
M3 - Article
C2 - 20459638
AN - SCOPUS:77951952760
SN - 1471-213X
VL - 10
JO - BMC Developmental Biology
JF - BMC Developmental Biology
M1 - 47
ER -