Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF1α). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.
- adenylate cyclase
- retinal pigment epithelium
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience