Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro

Zvi Friedman, Sean F. Hackett, Peter A. Campochiaro

Research output: Contribution to journalArticle

Abstract

Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.

Original languageEnglish (US)
Pages (from-to)471-479
Number of pages9
JournalExperimental eye research
Volume44
Issue number4
DOIs
StatePublished - 1987
Externally publishedYes

Keywords

  • adenylate cyclase
  • beta-adrenergies
  • cAMP
  • prostaglandins
  • retinal pigment epithelium

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro'. Together they form a unique fingerprint.

  • Cite this