Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro

Zvi Friedman; Sean F. Hackett; Peter A. Campochiaro

doi:10.1016/S0014-4835(87)80158-6

Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro

Zvi Friedman, Sean F. Hackett, Peter A. Campochiaro

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32 Scopus citations

Abstract

Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE₁ and PGE₂, but not PGF_1α). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.

Original language	English (US)
Pages (from-to)	471-479
Number of pages	9
Journal	Experimental eye research
Volume	44
Issue number	4
DOIs	https://doi.org/10.1016/S0014-4835(87)80158-6
State	Published - 1987
Externally published	Yes

Keywords

adenylate cyclase
beta-adrenergies
cAMP
prostaglandins
retinal pigment epithelium

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro",

abstract = "Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF1α). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.",

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AU - Friedman, Zvi

AU - Hackett, Sean F.

AU - Campochiaro, Peter A.

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N2 - Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF1α). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.

AB - Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (l-isoproterenol ≥ l-epinephrine > l-norepinephrine) and some prostaglandins (PGE1 and PGE2, but not PGF1α). The adrenergic response appears to be mediated by beta-2 receptors. No inhibitory ligands could be demonstrated. Its characteristics, which are similar to functional adenylate cyclase complexes in other mammalian cells, and its selective and sensitive agonist responsiveness, suggest a possible physiologic role in the regulation of human retinal pigment epithelial-cell function.

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KW - beta-adrenergies

KW - cAMP

KW - prostaglandins

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Characterization of adenylate cyclase in human retinal pigment epithelial cells in vitro

Abstract

Keywords

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