Characterization of a renal cell carcinoma cell line derived from a human bone metastasis and establishment of an experimental nude mouse model

Kristy L. Weber, Sen Pathak, Asha S. Multani, Janet E. Price

Research output: Contribution to journalArticle

Abstract

Purpose: We provide the necessary tools to study the biology of bone metastasis using renal cell carcinoma as a model. A relevant renal cell carcinoma cell line developed from a human bone metastasis is described and an experimental model in the nude mouse was established. Materials and Methods: The novel cell line RBM1 was developed from bone metastasis from a patient with renal cell carcinoma. In vitro methods used to study the cell line included karyotype analysis, reverse-transcriptase polymerase chain reaction, ribonuclease protection assay and Western blot analysis. An intratibial injection model in the nude mouse resulted in bone lesions similar to those in human patients. Results: Chromosomal analysis of this cell line revealed characteristic cytogenetic abnormalities common in renal cell carcinoma. RBM1 cells expressed parathyroid hormone parathyroid hormone related peptide, interleukin-6 and macrophage colony-stimulating factor, which are cytokines involved in osteoclast activation and subsequent bone resorption. Western blot analysis revealed the presence of high levels of epidermal growth factor receptor and c-MET. Reproducible osteolytic lesions developed in the nude mouse after injecting 1 × 106 cells into the tibia. Conclusions: This cell line and animal model may allow further study of the biology and mechanism of renal cell carcinoma bone metastasis.

Original languageEnglish (US)
Pages (from-to)774-779
Number of pages6
JournalJournal of Urology
Volume168
Issue number2
DOIs
StatePublished - Jan 1 2002

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Keywords

  • Bone and bones
  • Carcinoma, renal cell
  • Kidney
  • Mice, nude
  • Neoplasm metastasis

ASJC Scopus subject areas

  • Urology

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