Characterization of a novel metastatic prostate cancer cell line of LNCaP origin

Mark A. Castanares, Ben T. Copeland, Wasim H. Chowdhury, Minzhi M. Liu, Ronald Rodriguez, Martin Gilbert Pomper, Shawn Lupold, Catherine Foss

Research output: Contribution to journalArticle

Abstract

BACKGROUND The LNCaP cell line was originally isolated from the lymph node of a patient with metastatic prostate cancer. Many cell lines have been derived from LNCaP by selective pressures to study different aspects of prostate cancer progression. When injected subcutaneously into male athymic nude mice, LNCaP and its derivatives rarely metastasize. METHODS Here, we describe the characteristics of a new LNCaP derivative, JHU-LNCaP-SM, which was generated by long term passage in normal cell culture conditions. RESULTS Short tandem repeat (STR) analysis and genomic sequencing verified JHU-LNCaP-SM derivation from parental LNCaP cells. JHU-LNCaP-SM cells express the same mutated androgen receptor (AR) but unlike LNCaP, are no longer androgen dependent for growth. The cells demonstrate an attenuated androgen responsiveness in transcriptional assays and retain androgen sensitive expression of PSA, AR, and PSMA. Unlike parental LNCaP, JHU-LNCaP-SM cells quickly form subcutaneous tumors in male athymic nude mice, reliably metastasize to the lymph nodes and display a striking intra-tumoral and spreading hemorrhagic phenotype as tumor xenografts. CONCLUSIONS The JHU-LNCaP-SM cell line is a new isolate of LNCaP, which facilitates practical, preclinical studies of spontaneous metastasis of prostate cancer through lymphatic tissues.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalProstate
Volume76
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Nude Mice
Prostatic Neoplasms
Androgens
Cell Line
Androgen Receptors
Lymph Nodes
Lymphoid Tissue
Heterografts
Microsatellite Repeats
Neoplasms
Cell Culture Techniques
Neoplasm Metastasis
Phenotype
Growth

Keywords

  • androgen
  • JHU-LNCaP-SM
  • lymph node
  • metastasis
  • PSMA

ASJC Scopus subject areas

  • Urology
  • Oncology

Cite this

Castanares, M. A., Copeland, B. T., Chowdhury, W. H., Liu, M. M., Rodriguez, R., Pomper, M. G., ... Foss, C. (2016). Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. Prostate, 76(2), 215-225. https://doi.org/10.1002/pros.23115

Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. / Castanares, Mark A.; Copeland, Ben T.; Chowdhury, Wasim H.; Liu, Minzhi M.; Rodriguez, Ronald; Pomper, Martin Gilbert; Lupold, Shawn; Foss, Catherine.

In: Prostate, Vol. 76, No. 2, 01.02.2016, p. 215-225.

Research output: Contribution to journalArticle

Castanares, MA, Copeland, BT, Chowdhury, WH, Liu, MM, Rodriguez, R, Pomper, MG, Lupold, S & Foss, C 2016, 'Characterization of a novel metastatic prostate cancer cell line of LNCaP origin', Prostate, vol. 76, no. 2, pp. 215-225. https://doi.org/10.1002/pros.23115
Castanares MA, Copeland BT, Chowdhury WH, Liu MM, Rodriguez R, Pomper MG et al. Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. Prostate. 2016 Feb 1;76(2):215-225. https://doi.org/10.1002/pros.23115
Castanares, Mark A. ; Copeland, Ben T. ; Chowdhury, Wasim H. ; Liu, Minzhi M. ; Rodriguez, Ronald ; Pomper, Martin Gilbert ; Lupold, Shawn ; Foss, Catherine. / Characterization of a novel metastatic prostate cancer cell line of LNCaP origin. In: Prostate. 2016 ; Vol. 76, No. 2. pp. 215-225.
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