Characterization of a novel high-dose ovalbumin-induced murine model of allergic sinonasal inflammation

Michelle Mendiola, Anuj Tharakan, Mengfei Chen, Tomefa Asempa, Andrew P. Lane, Murugappan Ramanathan

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Few efficacious topical therapies exist for chronic rhinosinusitis (CRS). The lack of a reproducible mouse model of CRS limits the pilot testing of potential novel anti-inflammatory therapies. Although the ovalbumin-induced mouse model of sinonasal inflammation is commonly used, it is difficult to reproduce and can generate variable histologic results. In this study, we explore a variation of this model in different strains of mice and explore various inflammatory cytokines as reproducible molecular markers of inflammation. Methods: Allergic sinonasal inflammation was generated in BALB/c and C57BL/6 mice using intraperitoneal high-dose injections of ovalbumin (Ova; Sigma Chemical Co.) followed by 10 days of high-dose intranasal sensitization. Real-time polymerase chain reaction (RT-PCR) for eotaxin, interleukin 4 (IL-4), and IL-13 were measured from sinonasal mucosa. We also pilot tested a known topical budesonide to characterize the anti-inflammatory response. Histological sections were analyzed for epithelial thickness and eosinophilia. Results: Both BALB/c and C57BL/6 mice consistently showed increases in T helper 2 (Th2) cytokines after sensitization with high-dose Ova (p < 0.0001) when compared to controls. There were also significant increases in epithelial thickening in Ova-sensitized mice and eosinophilia in both BALB/c and C57BL/6 strains. In addition, topical budesonide significantly reduced anti-inflammatory cytokines, eosinophilia, and epithelial thickness. Conclusion: Our variation of the ovalbumin-induced mouse model of sinonasal inflammation in both BALB/c and C57BL/6 mice provides an efficacious model for testing potential topical anti-inflammatory therapies for CRS. The utilization of sinonasal mucosal Th2 cytokines along with histologic markers provides a consistent and quantifiable marker of inflammation in assessing the efficacy of candidate drugs.

Original languageEnglish (US)
Pages (from-to)964-972
Number of pages9
JournalInternational Forum of Allergy and Rhinology
Volume6
Issue number9
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Keywords

  • allergic rhinosinusitis
  • eosinophilia
  • mouse model
  • ovalbumin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Fingerprint

Dive into the research topics of 'Characterization of a novel high-dose ovalbumin-induced murine model of allergic sinonasal inflammation'. Together they form a unique fingerprint.

Cite this