Characterization of a murine model of endotoxin-induced acute lung injury

Koroush Kabir, Jean Pierre Gelinas, Meihong Chen, Dongfen Chen, Dexin Zhang, Xiaoxing Luo, Jing Hua Yang, Darryl Carter, Reuven Rabinovici

Research output: Contribution to journalArticle

Abstract

Endotoxin-induced microvascular lung injury in mice is a commonly used experimental model of the acute respiratory distress syndrome (ARDS). The present paper aimed to characterize this popular model in a comprehensive and systematic fashion. Male C57bl/6 mice (n = 5) were administered an LD55 dose of E. coli endotoxin (15 mg/kg, i.p.), and lungs were harvested at several time points and evaluated for injury as well as for expression of a variety of inflammatory mediators. Endotoxin induced many features characteristic of acute microvascular lung injury. These included early (1-2 h) expression of inflammatory mediators (IL-1α, IL-1β, IL-4, IL-6, IL-10, TNF-α, interferon-α, interferon-γ, and MCP-1) and leukocyte accumulation in lung tissue (lung myeloperoxidase activity 18.5 ± 7.8 U/g tissue, P <0.05), followed by pulmonary edema (lung water content index 17.4% ± 2.5%, P <0.05) and mortality. Histopathological evaluation of lung tissue was compatible with these findings. The characterization of this murine model of endotoxin-induced microvascular injury will facilitate its utilization in ARDS research.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalShock
Volume17
Issue number4
StatePublished - Apr 2002
Externally publishedYes

Keywords

  • ARDS
  • Cytokines
  • Endotoxin
  • Interferon-γ
  • Mice

ASJC Scopus subject areas

  • Physiology
  • Critical Care and Intensive Care Medicine

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  • Cite this

    Kabir, K., Gelinas, J. P., Chen, M., Chen, D., Zhang, D., Luo, X., Yang, J. H., Carter, D., & Rabinovici, R. (2002). Characterization of a murine model of endotoxin-induced acute lung injury. Shock, 17(4), 300-303.