Characterization of a MEN1 ortholog from Drosophila melanogaster

Siradanahalli C. Guru, Nijaguna B. Prasad, Eun J. Shin, Kirugaval Hemavathy, Jining Lu, Y. Tony Ip, Sunita K. Agarwal, Stephen J. Marx, Allen M. Spiegel, Francis S. Collins, Brian Oliver, Settara C. Chandrasekharappa

Research output: Contribution to journalArticle

Abstract

Multiple endocrine neoplasia type 1 (MEN1) is a familial cancer syndrome characterized by tumors of the parathyroid, entero-pancreatic neuroendocrine and pituitary tissues and caused by inactivating mutations in the MEN1 gene. Menin, the 610-amino acid nuclear protein encoded by MEN1, binds to the transcription factor JunD and can repress JunD-induced transcription. We report here the identification of a MEN1 ortholog in Drosophila melanogaster, Menin1, that encodes a 763 amino acid protein sharing 46% identity with human menin. Additionally, 69% of the missense mutations and in-frame deletions reported in MEN1 patients appear in amino acid residues that are identical in the Drosophila and human protein, suggesting the importance of the conserved regions. Drosophila Menin1 gene transcripts use alternative polyadenylation sites resulting in 4.3 and 5-kb messages. The 4.3-kb transcript appears to be largely maternal, while the 5-kb transcript appears mainly zygotic. The binding of Drosophila menin to human JunD or Drosophila Jun could not be demonstrated by the yeast two-hybrid analysis. The identification of the MEN1 ortholog from Drosophila melanogaster will provide an opportunity to utilize Drosophila genetics to enhance our understanding of the function of human menin.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalGene
Volume263
Issue number1-2
DOIs
StatePublished - Jan 24 2001
Externally publishedYes

Fingerprint

Multiple Endocrine Neoplasia Type 1
Drosophila melanogaster
Drosophila
Amino Acids
Polyadenylation
Missense Mutation
Nuclear Proteins
Genes
Neoplasms
Transcription Factors
Yeasts
Mothers
Mutation
Proteins

Keywords

  • DJun
  • JunD
  • MEN1
  • Menin
  • Tumor suppressor

ASJC Scopus subject areas

  • Genetics

Cite this

Guru, S. C., Prasad, N. B., Shin, E. J., Hemavathy, K., Lu, J., Ip, Y. T., ... Chandrasekharappa, S. C. (2001). Characterization of a MEN1 ortholog from Drosophila melanogaster. Gene, 263(1-2), 31-38. https://doi.org/10.1016/S0378-1119(00)00562-X

Characterization of a MEN1 ortholog from Drosophila melanogaster. / Guru, Siradanahalli C.; Prasad, Nijaguna B.; Shin, Eun J.; Hemavathy, Kirugaval; Lu, Jining; Ip, Y. Tony; Agarwal, Sunita K.; Marx, Stephen J.; Spiegel, Allen M.; Collins, Francis S.; Oliver, Brian; Chandrasekharappa, Settara C.

In: Gene, Vol. 263, No. 1-2, 24.01.2001, p. 31-38.

Research output: Contribution to journalArticle

Guru, SC, Prasad, NB, Shin, EJ, Hemavathy, K, Lu, J, Ip, YT, Agarwal, SK, Marx, SJ, Spiegel, AM, Collins, FS, Oliver, B & Chandrasekharappa, SC 2001, 'Characterization of a MEN1 ortholog from Drosophila melanogaster', Gene, vol. 263, no. 1-2, pp. 31-38. https://doi.org/10.1016/S0378-1119(00)00562-X
Guru SC, Prasad NB, Shin EJ, Hemavathy K, Lu J, Ip YT et al. Characterization of a MEN1 ortholog from Drosophila melanogaster. Gene. 2001 Jan 24;263(1-2):31-38. https://doi.org/10.1016/S0378-1119(00)00562-X
Guru, Siradanahalli C. ; Prasad, Nijaguna B. ; Shin, Eun J. ; Hemavathy, Kirugaval ; Lu, Jining ; Ip, Y. Tony ; Agarwal, Sunita K. ; Marx, Stephen J. ; Spiegel, Allen M. ; Collins, Francis S. ; Oliver, Brian ; Chandrasekharappa, Settara C. / Characterization of a MEN1 ortholog from Drosophila melanogaster. In: Gene. 2001 ; Vol. 263, No. 1-2. pp. 31-38.
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