Abstract
Borna disease virus (BDV) infects cells of the nervous system in a wide range of species. Previous work suggests that there are differences in BDV replication in neuronal cells and glial cells. Many neurons are lysed by the immunopathologic response to BDV; lysis of dentate gyrus neurons in the absence of encephalitis is seen in rats inoculated with BDV as neonates. In contrast, persistently BDV-infected astrocytes increase over the course of BDV infection. Therefore, we compared BDV replication in neuronal (SK-N-SH and SK-N-SHEP) and astrocytic (C6) cell lines. While SK-N-SH cells produced more infectious virions per cell, the C6 cells contained more BDV proteins and RNA. BDV sequences in the supernatants of both cell types were identified, despite low titers of infectious virus, suggesting the release of incomplete virions into the medium. C6 cells secreted a factor or factors into the medium that enhanced the production of BDV proteins and RNA in other cell lines. In addition, nerve growth factor treatment produced the same enhancement. Thus, BDV replication in certain neural cells in vitro may be linked to the production of cell-specific factors which affect viral replication.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1453-1460 |
| Number of pages | 8 |
| Journal | Journal of Virology |
| Volume | 67 |
| Issue number | 3 |
| State | Published - Mar 1993 |
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ASJC Scopus subject areas
- Immunology
Cite this
Characterization of a glial cell line persistently infected with borna disease virus (BDV) : Influence of neurotrophic factors on BDV protein and RNA expression. / Carbone, Kathryn M.; Rubin, Steven A.; Sierra-Honigmann, Ana M.; Lederman, Howard M.
In: Journal of Virology, Vol. 67, No. 3, 03.1993, p. 1453-1460.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of a glial cell line persistently infected with borna disease virus (BDV)
T2 - Influence of neurotrophic factors on BDV protein and RNA expression
AU - Carbone, Kathryn M.
AU - Rubin, Steven A.
AU - Sierra-Honigmann, Ana M.
AU - Lederman, Howard M
PY - 1993/3
Y1 - 1993/3
N2 - Borna disease virus (BDV) infects cells of the nervous system in a wide range of species. Previous work suggests that there are differences in BDV replication in neuronal cells and glial cells. Many neurons are lysed by the immunopathologic response to BDV; lysis of dentate gyrus neurons in the absence of encephalitis is seen in rats inoculated with BDV as neonates. In contrast, persistently BDV-infected astrocytes increase over the course of BDV infection. Therefore, we compared BDV replication in neuronal (SK-N-SH and SK-N-SHEP) and astrocytic (C6) cell lines. While SK-N-SH cells produced more infectious virions per cell, the C6 cells contained more BDV proteins and RNA. BDV sequences in the supernatants of both cell types were identified, despite low titers of infectious virus, suggesting the release of incomplete virions into the medium. C6 cells secreted a factor or factors into the medium that enhanced the production of BDV proteins and RNA in other cell lines. In addition, nerve growth factor treatment produced the same enhancement. Thus, BDV replication in certain neural cells in vitro may be linked to the production of cell-specific factors which affect viral replication.
AB - Borna disease virus (BDV) infects cells of the nervous system in a wide range of species. Previous work suggests that there are differences in BDV replication in neuronal cells and glial cells. Many neurons are lysed by the immunopathologic response to BDV; lysis of dentate gyrus neurons in the absence of encephalitis is seen in rats inoculated with BDV as neonates. In contrast, persistently BDV-infected astrocytes increase over the course of BDV infection. Therefore, we compared BDV replication in neuronal (SK-N-SH and SK-N-SHEP) and astrocytic (C6) cell lines. While SK-N-SH cells produced more infectious virions per cell, the C6 cells contained more BDV proteins and RNA. BDV sequences in the supernatants of both cell types were identified, despite low titers of infectious virus, suggesting the release of incomplete virions into the medium. C6 cells secreted a factor or factors into the medium that enhanced the production of BDV proteins and RNA in other cell lines. In addition, nerve growth factor treatment produced the same enhancement. Thus, BDV replication in certain neural cells in vitro may be linked to the production of cell-specific factors which affect viral replication.
UR - http://www.scopus.com/inward/record.url?scp=0027400771&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027400771&partnerID=8YFLogxK
M3 - Article
C2 - 8437223
AN - SCOPUS:0027400771
VL - 67
SP - 1453
EP - 1460
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 3
ER -