Characterization of a glial cell line persistently infected with borna disease virus (BDV)

Influence of neurotrophic factors on BDV protein and RNA expression

Kathryn M. Carbone, Steven A. Rubin, Ana M. Sierra-Honigmann, Howard M Lederman

Research output: Contribution to journalArticle

Abstract

Borna disease virus (BDV) infects cells of the nervous system in a wide range of species. Previous work suggests that there are differences in BDV replication in neuronal cells and glial cells. Many neurons are lysed by the immunopathologic response to BDV; lysis of dentate gyrus neurons in the absence of encephalitis is seen in rats inoculated with BDV as neonates. In contrast, persistently BDV-infected astrocytes increase over the course of BDV infection. Therefore, we compared BDV replication in neuronal (SK-N-SH and SK-N-SHEP) and astrocytic (C6) cell lines. While SK-N-SH cells produced more infectious virions per cell, the C6 cells contained more BDV proteins and RNA. BDV sequences in the supernatants of both cell types were identified, despite low titers of infectious virus, suggesting the release of incomplete virions into the medium. C6 cells secreted a factor or factors into the medium that enhanced the production of BDV proteins and RNA in other cell lines. In addition, nerve growth factor treatment produced the same enhancement. Thus, BDV replication in certain neural cells in vitro may be linked to the production of cell-specific factors which affect viral replication.

Original languageEnglish (US)
Pages (from-to)1453-1460
Number of pages8
JournalJournal of Virology
Volume67
Issue number3
StatePublished - Mar 1993

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Borna disease virus
neurotrophins
Nerve Growth Factors
neuroglia
Neuroglia
cell lines
RNA
Cell Line
Proteins
proteins
virus replication
Virus Replication
neurons
cells
virion
Virion
Virus Release
Neurons
nerve growth factor
astrocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of a glial cell line persistently infected with borna disease virus (BDV) : Influence of neurotrophic factors on BDV protein and RNA expression. / Carbone, Kathryn M.; Rubin, Steven A.; Sierra-Honigmann, Ana M.; Lederman, Howard M.

In: Journal of Virology, Vol. 67, No. 3, 03.1993, p. 1453-1460.

Research output: Contribution to journalArticle

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