Characterization of a gene coamplified with ki-ras in Y1 murine adrenal carcinoma cells that codes for a putative membrane protein

Alan F. Scott, Andrew Elizaga, James Morrell, Andrew Bergen, Margaret B. Penno

Research output: Contribution to journalArticlepeer-review

Abstract

We report the sequence and genomic organization of a gene linked to Ki-ras in the mouse and coamplified in Y1 murine adrenal carcinoma cells. The entire 4.4-kb cDNA sequence as well as promoter and splice sites for each of the three exons was determined. The gene, designated KRAG (Ki-ras-associated gene) has a CG-rich first exon and promoter region and a long 3′ untranslated region and encodes 216 amino acids. The putative 23.9-kDa protein has four potential transmembrane hydrophobic domains. The hydropathy plot resembles that of certain tumor-associated antigens, including CO-029 and ME491. A potential human homologue, EST05985, was identified and provisionally mapped to human chromosome 12, a chromosome syntenic to mouse chromosome 6, the previously mapped location of KRAG.

Original languageEnglish (US)
Pages (from-to)227-230
Number of pages4
JournalGenomics
Volume20
Issue number2
DOIs
StatePublished - Mar 15 1994

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Characterization of a gene coamplified with ki-ras in Y1 murine adrenal carcinoma cells that codes for a putative membrane protein'. Together they form a unique fingerprint.

Cite this