Characterization of a chemokine receptor-related gene in human herpesvirus 8 and its expression in Kaposi's sarcoma

Hong Guang Guo, Philip Browning, John Nicholas, Gary Selwyn Hayward, Erwin Tschachler, Yi Wen Jiang, Mariola Sadowska, Mark Raffeld, Sandra Colombini, Robert C. Gallo, Marvin S. Reitz

Research output: Contribution to journalArticle

Abstract

Human herpesvirus 8 (HHV-8) is a recently discovered virus that is highly associated with Kaposi's sarcoma (KS) and AIDS-associated body cavity lymphomas, although it is also found in some normal individuals. HHV-8 is related by nucleotide sequence homology to herpesvirus saimiri (HVS), which causes T cell lymphomas in some New World monkeys, and to Epstein-Barr virus (EBV), a human herpesvirus linked etiologically with Burkitt's lymphoma and nasopharyngeal carcinoma. We report that, like HVS but unlike EBV, HHV-8 contains a gene (ORF74) with significant sequence homology to the high-affinity IL-8 receptor, a member of the α (CXC) chemokine receptor family of transmembrane G protein-coupled receptors. We also show by reverse transcription PCR that the chemokine receptor-related HHV-8 gene is detectable in some RNA samples from KS tissue, and that its expression varies independently from that of ORF26, a minor capsid protein. The presence of a potential chemokine receptor in HHV-8 and its expression in KS tissue suggests that it may be important in the regulation of viral gene expression and may play a role in the etiology of KS and AIDS-related body cavity lymphomas.

Original languageEnglish (US)
Pages (from-to)371-378
Number of pages8
JournalVirology
Volume228
Issue number2
DOIs
StatePublished - Feb 17 1997

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Human Herpesvirus 8
Chemokine Receptors
Kaposi's Sarcoma
Saimiriine Herpesvirus 2
Genes
Sequence Homology
Human Herpesvirus 4
Viral Gene Expression Regulation
Lymphoma
CXCR Receptors
Interleukin-8 Receptors
Platyrrhini
Burkitt Lymphoma
T-Cell Lymphoma
Herpesviridae
Capsid Proteins
G-Protein-Coupled Receptors
Reverse Transcription
Acquired Immunodeficiency Syndrome
RNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Characterization of a chemokine receptor-related gene in human herpesvirus 8 and its expression in Kaposi's sarcoma. / Guo, Hong Guang; Browning, Philip; Nicholas, John; Hayward, Gary Selwyn; Tschachler, Erwin; Jiang, Yi Wen; Sadowska, Mariola; Raffeld, Mark; Colombini, Sandra; Gallo, Robert C.; Reitz, Marvin S.

In: Virology, Vol. 228, No. 2, 17.02.1997, p. 371-378.

Research output: Contribution to journalArticle

Guo, HG, Browning, P, Nicholas, J, Hayward, GS, Tschachler, E, Jiang, YW, Sadowska, M, Raffeld, M, Colombini, S, Gallo, RC & Reitz, MS 1997, 'Characterization of a chemokine receptor-related gene in human herpesvirus 8 and its expression in Kaposi's sarcoma', Virology, vol. 228, no. 2, pp. 371-378. https://doi.org/10.1006/viro.1996.8386
Guo, Hong Guang ; Browning, Philip ; Nicholas, John ; Hayward, Gary Selwyn ; Tschachler, Erwin ; Jiang, Yi Wen ; Sadowska, Mariola ; Raffeld, Mark ; Colombini, Sandra ; Gallo, Robert C. ; Reitz, Marvin S. / Characterization of a chemokine receptor-related gene in human herpesvirus 8 and its expression in Kaposi's sarcoma. In: Virology. 1997 ; Vol. 228, No. 2. pp. 371-378.
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