Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti

M. J. Edwards, L. A. Moskalyk, M. Donelly-Doman, M. Vlaskova, F. G. Noriega, V. K. Walker, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalInsect Molecular Biology
Volume9
Issue number1
DOIs
StatePublished - Feb 2000
Externally publishedYes

Fingerprint

carboxypeptidase A
Carboxypeptidases A
Carboxypeptidases
carboxypeptidases
Aedes
messenger RNA
Aedes aegypti
Culicidae
Anopheles gambiae
Genes
Messenger RNA
pupal development
genes
gene induction
blood meal
Gene expression
midgut
larval development
Trypsin
in situ hybridization

Keywords

  • Aedes aegypti
  • Carboxypeptidase A
  • Mosquito

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Edwards, M. J., Moskalyk, L. A., Donelly-Doman, M., Vlaskova, M., Noriega, F. G., Walker, V. K., & Jacobs-Lorena, M. (2000). Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti. Insect Molecular Biology, 9(1), 33-38. https://doi.org/10.1046/j.1365-2583.2000.00159.x

Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti. / Edwards, M. J.; Moskalyk, L. A.; Donelly-Doman, M.; Vlaskova, M.; Noriega, F. G.; Walker, V. K.; Jacobs-Lorena, Marcelo.

In: Insect Molecular Biology, Vol. 9, No. 1, 02.2000, p. 33-38.

Research output: Contribution to journalArticle

Edwards, MJ, Moskalyk, LA, Donelly-Doman, M, Vlaskova, M, Noriega, FG, Walker, VK & Jacobs-Lorena, M 2000, 'Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti', Insect Molecular Biology, vol. 9, no. 1, pp. 33-38. https://doi.org/10.1046/j.1365-2583.2000.00159.x
Edwards MJ, Moskalyk LA, Donelly-Doman M, Vlaskova M, Noriega FG, Walker VK et al. Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti. Insect Molecular Biology. 2000 Feb;9(1):33-38. https://doi.org/10.1046/j.1365-2583.2000.00159.x
Edwards, M. J. ; Moskalyk, L. A. ; Donelly-Doman, M. ; Vlaskova, M. ; Noriega, F. G. ; Walker, V. K. ; Jacobs-Lorena, Marcelo. / Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti. In: Insect Molecular Biology. 2000 ; Vol. 9, No. 1. pp. 33-38.
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