Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti

M. J. Edwards, L. A. Moskalyk, M. Donelly-Doman, M. Vlaskova, F. G. Noriega, V. K. Walker, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticlepeer-review


A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.

Original languageEnglish (US)
Pages (from-to)33-38
Number of pages6
JournalInsect molecular biology
Issue number1
StatePublished - Feb 2000
Externally publishedYes


  • Aedes aegypti
  • Carboxypeptidase A
  • Mosquito

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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