Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti

M. J. Edwards; L. A. Moskalyk; M. Donelly-Doman; M. Vlaskova; F. G. Noriega; V. K. Walker; Marcelo Jacobs-Lorena

doi:10.1046/j.1365-2583.2000.00159.x

Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti

M. J. Edwards, L. A. Moskalyk, M. Donelly-Doman, M. Vlaskova, F. G. Noriega, V. K. Walker, Marcelo Jacobs-Lorena

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.

Original language	English (US)
Pages (from-to)	33-38
Number of pages	6
Journal	Insect molecular biology
Volume	9
Issue number	1
DOIs	https://doi.org/10.1046/j.1365-2583.2000.00159.x
State	Published - Feb 2000
Externally published	Yes

Keywords

Aedes aegypti
Carboxypeptidase A
Mosquito

ASJC Scopus subject areas

Molecular Biology
Genetics
Insect Science

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10.1046/j.1365-2583.2000.00159.x

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title = "Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti",

abstract = "A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.",

keywords = "Aedes aegypti, Carboxypeptidase A, Mosquito",

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year = "2000",

month = feb,

doi = "10.1046/j.1365-2583.2000.00159.x",

language = "English (US)",

volume = "9",

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AU - Edwards, M. J.

AU - Moskalyk, L. A.

AU - Donelly-Doman, M.

AU - Vlaskova, M.

AU - Noriega, F. G.

AU - Walker, V. K.

AU - Jacobs-Lorena, Marcelo

PY - 2000/2

Y1 - 2000/2

N2 - A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.

AB - A gut-specific carboxypeptidase A gene (AeCPA) from the mosquito, Aedes aegypti, was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≃ 16-24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.

KW - Aedes aegypti

KW - Carboxypeptidase A

KW - Mosquito

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Characterization of a carboxypeptidase a gene from the mosquito, Aedes aegypti

Abstract

Keywords

ASJC Scopus subject areas

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