Characterization, cloning and expression of the 67-kDa annexin from chicken growth plate cartilage matrix vesicles

Xu Cao, Brian R. Genge, Licia N.Y. Wu, William R. Buzzi, Richard M. Showman, Roy E. Wuthier

Research output: Contribution to journalArticlepeer-review

Abstract

Analysis of a 67 kDa lipid-dependent Ca2+-binding protein from avian matrix vesicles revealed amino acid sequences homologous to mammalian annexin VI. PCR methods were used to identify a clone from an avian cDNA library that contained a full length copy of the 67-kDa annexin cDNA. This was restriction mapped, subcloned and sequenced. The cDNA sequence of the open reading frame showed 70 percent identity to that of murine annexin VI; the predicted amino acid sequence, 78 percent identity. There was no homology in the 5’- and 3’-untranslated regions. A plasmid was constructed that overexpresses the intact chicken 67-kDa matrix vesicle annexin in E. coli DH5α in high yield; the physicochemical properties and the amino terminal sequence of the expressed protein exactly matched those of the native protein.

Original languageEnglish (US)
Pages (from-to)556-561
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume197
Issue number2
DOIs
StatePublished - Dec 15 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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