@article{5bda41752272405b9a3810aadd110adf,
title = "Characterization and in vitro testing of newly isolated lytic bacteriophages for the biocontrol of Pseudomonas aeruginosa",
abstract = "Aim: Two lytic phages were isolated using P. aeruginosa DSM19880 as host and fully characterized. Materials & methods: Phages were characterized physicochemically, biologically and genomically. Results & conclusion: Host range analysis revealed that the phages also infect some multidrug-resistant (MDR) P. aeruginosa clinical isolates. Increasing MOI from 1 to 1000 significantly increased phage efficiency and retarded bacteria regrowth, but phage ph0034 (reduction of 7.5 log CFU/ml) was more effective than phage ph0031 (reduction of 5.1 log CFU/ml) after 24 h. Both phages belong to Myoviridae family. Genome sequencing of phages ph0031 and ph0034 showed that they do not carry toxin, virulence, antibiotic resistance and integrase genes. The results obtained are highly relevant in the actual context of bacterial resistance to antibiotics.",
keywords = "bacteria-phage inactivation, bacteriophage, genomic structural features, multidrug-resistant P. aeruginosa, physicochemical/biological/genomic characterization",
author = "Harada, {Liliam K.} and Silva, {Erica C.} and Rossi, {Fernando P.N.} and Basilio Cieza and Oliveira, {Thais J.} and Carla Pereira and Geizecler Tomazetto and Silva, {Bianca B.} and Squina, {Fabio M.} and Vila, {Marta M.D.C.} and Setubal, {Jo{\~a}o C.} and Taekjip Ha and {Da Silva}, {Aline M.} and Balc{\~a}o, {Victor M.}",
note = "Funding Information: This project was funded by Fundac¸{\~a}o de Amparo {\`a} Pesquisa do Estado de S{\~a}o Paulo (FAPESP, S{\~a}o Paulo, Brazil) (FAPESP ref. no. 2016/08884–3 [Project PneumoPhageColor] and 2016/12234–4 [Project TransAppIL]), is gratefully acknowledged. Funding for Victor M Balc{\~a}o through a BPE grant from FAPESP (S{\~a}o Paulo, Brazil) (ref. no. 2018/05522–9, Project PsaPhageKill) is gratefully acknowledged. This work also received support from CNPq, National Council for Scientific and Technological Development Brazil, in the form of Research Productivity (PQ) fellowships granted to Victor M Balc{\~a}o (ref. no. 306113/2014–7 and 308208/2017–0), Jo{\~a}o Carlos Setubal (ref. no. 305961/2019–5) and Aline Maria da Silva (ref. no. 314701/2020–6). Fernando Rossi was supported by a fellowship from Coordination for the Improvement of Higher Education Personnel (CAPES). Carla Pereira was supported by a Junior Research contract (CEEC Individual/03974/2017), financed by the Portuguese Foundation for Science and Technology (FCT). Thanks are due to FCT/MCTES for the financial support to CESAM (UID/AMB/50017/2019), through national funds. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2022 Future Medicine Ltd.",
year = "2022",
month = jan,
doi = "10.2217/fmb-2021-0027",
language = "English (US)",
volume = "17",
pages = "111--141",
journal = "Future microbiology",
issn = "1746-0913",
publisher = "Future Medicine Ltd.",
number = "2",
}