Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes

Panpan Chen, Xiaoju Guan, Xingxing Zhao, Fenfen Chen, Jianying Yang, Yiyan Wang, Yue Hu, Qingquan Lian, Haolin Chen

Research output: Contribution to journalArticle

Abstract

It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.

Original languageEnglish (US)
Article number110449
JournalMolecular and Cellular Endocrinology
Volume493
DOIs
StatePublished - Aug 1 2019

Fingerprint

Leydig Cells
Stem cells
Testis
Stem Cells
Macrophages
Genes
Nestin
Integrins
Staining and Labeling

Keywords

  • CD51
  • Macrophage
  • Stem Leydig cells
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes. / Chen, Panpan; Guan, Xiaoju; Zhao, Xingxing; Chen, Fenfen; Yang, Jianying; Wang, Yiyan; Hu, Yue; Lian, Qingquan; Chen, Haolin.

In: Molecular and Cellular Endocrinology, Vol. 493, 110449, 01.08.2019.

Research output: Contribution to journalArticle

Chen, Panpan ; Guan, Xiaoju ; Zhao, Xingxing ; Chen, Fenfen ; Yang, Jianying ; Wang, Yiyan ; Hu, Yue ; Lian, Qingquan ; Chen, Haolin. / Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes. In: Molecular and Cellular Endocrinology. 2019 ; Vol. 493.
@article{77f5778007d8437a94d28b8636c9ed75,
title = "Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes",
abstract = "It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.",
keywords = "CD51, Macrophage, Stem Leydig cells, Testis, Testosterone",
author = "Panpan Chen and Xiaoju Guan and Xingxing Zhao and Fenfen Chen and Jianying Yang and Yiyan Wang and Yue Hu and Qingquan Lian and Haolin Chen",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.mce.2019.110449",
language = "English (US)",
volume = "493",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Characterization and differentiation of CD51+ Stem Leydig cells in adult mouse testes

AU - Chen, Panpan

AU - Guan, Xiaoju

AU - Zhao, Xingxing

AU - Chen, Fenfen

AU - Yang, Jianying

AU - Wang, Yiyan

AU - Hu, Yue

AU - Lian, Qingquan

AU - Chen, Haolin

PY - 2019/8/1

Y1 - 2019/8/1

N2 - It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.

AB - It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51+ cells are SLCs. In the present study, we found that CD51+ cells can be classified into two sub-groups, a weakly-staining group (CD51+) and a strongly-staining group (CD51++). The CD51+ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51++ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51+ cells, but not CD51++ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.

KW - CD51

KW - Macrophage

KW - Stem Leydig cells

KW - Testis

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=85066628744&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066628744&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2019.110449

DO - 10.1016/j.mce.2019.110449

M3 - Article

VL - 493

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

M1 - 110449

ER -