Characterization and cloning of T24, a Taenia solium antigen diagnostic for cysticercosis

Kathy Hancock, Sowmya Pattabhi, Fatima W. Whitfield, Melinda L. Yushak, William S. Lane, Hector H. Garcia, Armandoe Gonzalez, Robert H Gilman, Victor C W Tsang

Research output: Contribution to journalArticle

Abstract

The third and final diagnostic antigen of the lentil lectin purified glycoproteins (LLGP) extracted from the larval stage of Taenia solium has been characterized, cloned, and expressed. T24 is an integral membrane protein that belongs to the tetraspanin superfamily. It migrates at a position corresponding to 24-kDa and as a homodimer at 42-kDa. Antibodies from cysticercosis patients recognize secondary structure epitopes that are dependent upon correctly formed disulfide bonds. A portion of T24, the large, extracellular loop domain, was expressed in an immunologically reactive form in insect cells. When tested in a Western blot assay with a large battery of serum samples, this protein, T24H, has a sensitivity of 94% (101/107), for detecting cases of cysticercosis with two or more viable cysts, and a specificity of 98% (284/290). The identification and expression of T24H sets the stage for the development of an ELISA suitable for testing single samples and for large-scale serosurveys that is not dependent upon the isolation and purification of antigens from parasite materials.

Original languageEnglish (US)
Pages (from-to)109-117
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume147
Issue number1
DOIs
StatePublished - May 2006

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Keywords

  • Cysticercosis
  • Diagnostic antigen
  • Neurocysticercosis
  • Recombinant protein
  • Taenia solium
  • Western blot

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

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