Characteristics of Shigella sonnei infection of volunteers

Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances

C. Munoz, S. Baqar, L. Van de Verg, J. Thupari, S. Goldblum, J. G. Olson, D. N. Taylor, G. P. Heresi, J. R. Murphy

Research output: Contribution to journalArticle

Abstract

Shigella sonnei infection resulting from oral administration of 500 colony-forming units was followed in 11 volunteers with the objective of studying the immune response and pathogenesis. Characterization of infection included recording of signs and symptoms, excretion of S. sonnei in stool, measurement of humoral tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ, (IFN-γ), C-reactive protein, IL-2 receptor, soluble CD8, antibody-antigen complexes, and endotoxin. Measurements were also made of the immune response including lymphocytes secreting antibody to S. sonnei O antigen and serum antibody to this antigen. Six of the volunteers developed typical shigellosis with excretion of bacteria in stool and systemic signs and symptoms, three excreted bacteria but did not show illness, and two showed no evidence of infection or illness. Shigellosis was characterized by excretion in stool of S. sonnei beginning on average 1.3 days after ingestion. Excretion of S. sonnei (mean of time of the first positive cultures) was followed in sequence by the onset of increases in TNF-α (10 hr), liquid stools (14 hr), fever and dysentery (18 hr), IFN-γ (22 hr), and C reactive protein (34 hr). A S. sonnei-specific immune response was demonstrated somewhat later, between days 4 and 7 postinfection by antibody secreting cells, and between days 7 and 14 postinfection by humoral antibody. Shigellosis was not associated with increased humoral IL-1β, endotoxin, or antigen-antibody complexes.

Original languageEnglish (US)
Pages (from-to)47-54
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume53
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Shigella sonnei
Signs and Symptoms
Volunteers
Cytokines
Bacillary Dysentery
Infection
Antibodies
Interleukin-1
Endotoxins
C-Reactive Protein
Interferons
Tumor Necrosis Factor-alpha
CD8 Antigens
Bacteria
Antibody-Producing Cells
O Antigens
Dysentery
Interleukin-2 Receptors
Antigen-Antibody Complex
Oral Administration

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Characteristics of Shigella sonnei infection of volunteers : Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances. / Munoz, C.; Baqar, S.; Van de Verg, L.; Thupari, J.; Goldblum, S.; Olson, J. G.; Taylor, D. N.; Heresi, G. P.; Murphy, J. R.

In: American Journal of Tropical Medicine and Hygiene, Vol. 53, No. 1, 1995, p. 47-54.

Research output: Contribution to journalArticle

Munoz, C, Baqar, S, Van de Verg, L, Thupari, J, Goldblum, S, Olson, JG, Taylor, DN, Heresi, GP & Murphy, JR 1995, 'Characteristics of Shigella sonnei infection of volunteers: Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances', American Journal of Tropical Medicine and Hygiene, vol. 53, no. 1, pp. 47-54.
Munoz, C. ; Baqar, S. ; Van de Verg, L. ; Thupari, J. ; Goldblum, S. ; Olson, J. G. ; Taylor, D. N. ; Heresi, G. P. ; Murphy, J. R. / Characteristics of Shigella sonnei infection of volunteers : Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances. In: American Journal of Tropical Medicine and Hygiene. 1995 ; Vol. 53, No. 1. pp. 47-54.
@article{b6d34e795ee443b6b2065f71d07ef322,
title = "Characteristics of Shigella sonnei infection of volunteers: Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances",
abstract = "Shigella sonnei infection resulting from oral administration of 500 colony-forming units was followed in 11 volunteers with the objective of studying the immune response and pathogenesis. Characterization of infection included recording of signs and symptoms, excretion of S. sonnei in stool, measurement of humoral tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ, (IFN-γ), C-reactive protein, IL-2 receptor, soluble CD8, antibody-antigen complexes, and endotoxin. Measurements were also made of the immune response including lymphocytes secreting antibody to S. sonnei O antigen and serum antibody to this antigen. Six of the volunteers developed typical shigellosis with excretion of bacteria in stool and systemic signs and symptoms, three excreted bacteria but did not show illness, and two showed no evidence of infection or illness. Shigellosis was characterized by excretion in stool of S. sonnei beginning on average 1.3 days after ingestion. Excretion of S. sonnei (mean of time of the first positive cultures) was followed in sequence by the onset of increases in TNF-α (10 hr), liquid stools (14 hr), fever and dysentery (18 hr), IFN-γ (22 hr), and C reactive protein (34 hr). A S. sonnei-specific immune response was demonstrated somewhat later, between days 4 and 7 postinfection by antibody secreting cells, and between days 7 and 14 postinfection by humoral antibody. Shigellosis was not associated with increased humoral IL-1β, endotoxin, or antigen-antibody complexes.",
author = "C. Munoz and S. Baqar and {Van de Verg}, L. and J. Thupari and S. Goldblum and Olson, {J. G.} and Taylor, {D. N.} and Heresi, {G. P.} and Murphy, {J. R.}",
year = "1995",
language = "English (US)",
volume = "53",
pages = "47--54",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "1",

}

TY - JOUR

T1 - Characteristics of Shigella sonnei infection of volunteers

T2 - Signs, symptoms, immune responses, changes in selected cytokines and acute-phase substances

AU - Munoz, C.

AU - Baqar, S.

AU - Van de Verg, L.

AU - Thupari, J.

AU - Goldblum, S.

AU - Olson, J. G.

AU - Taylor, D. N.

AU - Heresi, G. P.

AU - Murphy, J. R.

PY - 1995

Y1 - 1995

N2 - Shigella sonnei infection resulting from oral administration of 500 colony-forming units was followed in 11 volunteers with the objective of studying the immune response and pathogenesis. Characterization of infection included recording of signs and symptoms, excretion of S. sonnei in stool, measurement of humoral tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ, (IFN-γ), C-reactive protein, IL-2 receptor, soluble CD8, antibody-antigen complexes, and endotoxin. Measurements were also made of the immune response including lymphocytes secreting antibody to S. sonnei O antigen and serum antibody to this antigen. Six of the volunteers developed typical shigellosis with excretion of bacteria in stool and systemic signs and symptoms, three excreted bacteria but did not show illness, and two showed no evidence of infection or illness. Shigellosis was characterized by excretion in stool of S. sonnei beginning on average 1.3 days after ingestion. Excretion of S. sonnei (mean of time of the first positive cultures) was followed in sequence by the onset of increases in TNF-α (10 hr), liquid stools (14 hr), fever and dysentery (18 hr), IFN-γ (22 hr), and C reactive protein (34 hr). A S. sonnei-specific immune response was demonstrated somewhat later, between days 4 and 7 postinfection by antibody secreting cells, and between days 7 and 14 postinfection by humoral antibody. Shigellosis was not associated with increased humoral IL-1β, endotoxin, or antigen-antibody complexes.

AB - Shigella sonnei infection resulting from oral administration of 500 colony-forming units was followed in 11 volunteers with the objective of studying the immune response and pathogenesis. Characterization of infection included recording of signs and symptoms, excretion of S. sonnei in stool, measurement of humoral tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ, (IFN-γ), C-reactive protein, IL-2 receptor, soluble CD8, antibody-antigen complexes, and endotoxin. Measurements were also made of the immune response including lymphocytes secreting antibody to S. sonnei O antigen and serum antibody to this antigen. Six of the volunteers developed typical shigellosis with excretion of bacteria in stool and systemic signs and symptoms, three excreted bacteria but did not show illness, and two showed no evidence of infection or illness. Shigellosis was characterized by excretion in stool of S. sonnei beginning on average 1.3 days after ingestion. Excretion of S. sonnei (mean of time of the first positive cultures) was followed in sequence by the onset of increases in TNF-α (10 hr), liquid stools (14 hr), fever and dysentery (18 hr), IFN-γ (22 hr), and C reactive protein (34 hr). A S. sonnei-specific immune response was demonstrated somewhat later, between days 4 and 7 postinfection by antibody secreting cells, and between days 7 and 14 postinfection by humoral antibody. Shigellosis was not associated with increased humoral IL-1β, endotoxin, or antigen-antibody complexes.

UR - http://www.scopus.com/inward/record.url?scp=0029013286&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029013286&partnerID=8YFLogxK

M3 - Article

VL - 53

SP - 47

EP - 54

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 1

ER -