TY - JOUR
T1 - Characteristics of a pandemic clone of O3:K6 and O4:K68 Vibrio parahaemolyticus isolated in Beira, Mozambique
AU - Ansaruzzaman, M.
AU - Chowdhury, Ashrafuzzaman
AU - Bhuiyan, Nurul A.
AU - Sultana, Marzia
AU - Safa, Ashrafus
AU - Lucas, Marcelino
AU - Von Seidlein, Lorenz
AU - Barreto, Avertino
AU - Chaignat, Claire Lise
AU - Sack, David A.
AU - Clemens, John D.
AU - Nair, G. Balakrish
AU - Seon, Young Choi
AU - Yoon, Seong Jeon
AU - Je, Hee Lee
AU - Hye, Ri Lee
AU - Chun, Jongsik
AU - Dong, Wook Kim
PY - 2008/12
Y1 - 2008/12
N2 - The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3:K6 and O4:K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O:K antigens and genotyped for toxR, tdh and trh genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3:K6 (n=38) and O4:K68 (n=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3:K6 and O4:K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3:K6 and O4:K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3:K58 (n=8), O4:K13 (n=6), O3:KUT (n=1) and O8:K41 (n=1) strains showed unique characteristics different from the pandemic clone.
AB - The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3:K6 and O4:K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O:K antigens and genotyped for toxR, tdh and trh genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3:K6 (n=38) and O4:K68 (n=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3:K6 and O4:K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3:K6 and O4:K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3:K58 (n=8), O4:K13 (n=6), O3:KUT (n=1) and O8:K41 (n=1) strains showed unique characteristics different from the pandemic clone.
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U2 - 10.1099/jmm.0.2008/004275-0
DO - 10.1099/jmm.0.2008/004275-0
M3 - Article
C2 - 19018020
AN - SCOPUS:57749184340
SN - 0022-2615
VL - 57
SP - 1502
EP - 1507
JO - Journal of medical microbiology
JF - Journal of medical microbiology
IS - 12
ER -