TY - JOUR
T1 - Character of azurophil and specific granules purified from human polymorphonuclear leukocytes
AU - Spitznagel, J. K.
AU - Dalldorf, F. G.
AU - Leffell, M. S.
AU - Folds, J. D.
AU - Welsh, I. R.
AU - Cooney, M. H.
AU - Martin, L. E.
PY - 1974/12/1
Y1 - 1974/12/1
N2 - Cytoplasmic granules of polymorphonuclear leukocytes (PMN) from blood resolve during velocity centrifugation into well separated bands I, II, and III (sedimentation increasing in that order). Band I comprises empty vesicles and 85% of the biochemically measured alkaline phosphatase of the whole PMN. Specific granules, which are peroxidase negative and contain 50% of the PMN lysozyme and 77% of the lactoferrin, constitute band II. Their average diameter is 0.13 μm. The azurophil granules, with average diameters of 0.3 μm constitute band III and contain 88% of the immunochemically measured myeloperoxidase, 84% of the neutral protease, and 46% of the lysozyme. Both specific granules and azurophils are bounded by membranes. Band III can be further resolved into 2 narrowly separable bands: IIIs (slow), which contains more myeloperoxidase, neutral protease, and β glucuronidase; and IIIf (fast), which contains lysozyme. Histochemically, both IIIs and IIIf granules contain peroxidase. Measured immunochemically, lactoferrin and myeloperoxidase, respectively, appear to afford unambiguous markers for specific and azurophil granules of PMN. Although various blood cells may contaminate suspensions of PMN, only specific granules of eosinophils created problems.
AB - Cytoplasmic granules of polymorphonuclear leukocytes (PMN) from blood resolve during velocity centrifugation into well separated bands I, II, and III (sedimentation increasing in that order). Band I comprises empty vesicles and 85% of the biochemically measured alkaline phosphatase of the whole PMN. Specific granules, which are peroxidase negative and contain 50% of the PMN lysozyme and 77% of the lactoferrin, constitute band II. Their average diameter is 0.13 μm. The azurophil granules, with average diameters of 0.3 μm constitute band III and contain 88% of the immunochemically measured myeloperoxidase, 84% of the neutral protease, and 46% of the lysozyme. Both specific granules and azurophils are bounded by membranes. Band III can be further resolved into 2 narrowly separable bands: IIIs (slow), which contains more myeloperoxidase, neutral protease, and β glucuronidase; and IIIf (fast), which contains lysozyme. Histochemically, both IIIs and IIIf granules contain peroxidase. Measured immunochemically, lactoferrin and myeloperoxidase, respectively, appear to afford unambiguous markers for specific and azurophil granules of PMN. Although various blood cells may contaminate suspensions of PMN, only specific granules of eosinophils created problems.
UR - http://www.scopus.com/inward/record.url?scp=0016212483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016212483&partnerID=8YFLogxK
M3 - Article
C2 - 4134220
AN - SCOPUS:0016212483
SN - 0023-6837
VL - 30
SP - 774
EP - 785
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 6
ER -