Chapter 3 A Simple Assay for Local Heating by Optical Tweezers

Research output: Contribution to journalArticle

Abstract

This chapter discusses local heating by optical tweezers. Because most optical tweezers have been custom-built, if not customized for particular applications, estimates of aqueous heating are likely to vary between apparatuses. The chapter presents a simple, direct method to estimate the local heating by optical tweezers that will be useful to all practitioners of the technology. A number of methods exist for monitoring the local temperature near the field of observation. Microthermocouples can be inserted near the field of view, but accidental irradiation of the thermocouple by the optical tweezers laser can cause temperature jumps more than 500°C, which vaporize the local media.

Original languageEnglish (US)
Pages (from-to)43-45
Number of pages3
JournalMethods in cell biology
Volume55
Issue numberC
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Chapter 3 A Simple Assay for Local Heating by Optical Tweezers'. Together they form a unique fingerprint.

  • Cite this