Chapter 14 Methods to Determine mRNA Half-Life in Saccharomyces cerevisiae

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Much of our understanding of eukaryotic mRNA decay has come from studies in budding yeast, Saccharomyces cerevisiae. The facile nature of genetic and biochemical manipulations in yeast has allowed detailed investigations into the mRNA decay pathway and the identification of critical factors required for each step. Discussed herein are standard protocols for measuring mRNA half-lives in yeast. It should be noted, however, that variations of these assays are possible. In addition, a few a priori considerations are addressed.

Original languageEnglish (US)
Title of host publicationRNA Turnover in Eukaryotes
Subtitle of host publicationNucleases, Pathways and Anaylsis of mRNA Decay
EditorsLynne Maquat, Megerditch Kiledjian
Pages267-284
Number of pages18
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume448
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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