TY - CHAP
T1 - Chapter 14 Methods to Determine mRNA Half-Life in Saccharomyces cerevisiae
AU - Coller, Jeff
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008
Y1 - 2008
N2 - Much of our understanding of eukaryotic mRNA decay has come from studies in budding yeast, Saccharomyces cerevisiae. The facile nature of genetic and biochemical manipulations in yeast has allowed detailed investigations into the mRNA decay pathway and the identification of critical factors required for each step. Discussed herein are standard protocols for measuring mRNA half-lives in yeast. It should be noted, however, that variations of these assays are possible. In addition, a few a priori considerations are addressed.
AB - Much of our understanding of eukaryotic mRNA decay has come from studies in budding yeast, Saccharomyces cerevisiae. The facile nature of genetic and biochemical manipulations in yeast has allowed detailed investigations into the mRNA decay pathway and the identification of critical factors required for each step. Discussed herein are standard protocols for measuring mRNA half-lives in yeast. It should be noted, however, that variations of these assays are possible. In addition, a few a priori considerations are addressed.
UR - http://www.scopus.com/inward/record.url?scp=57749207970&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57749207970&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(08)02614-1
DO - 10.1016/S0076-6879(08)02614-1
M3 - Chapter
C2 - 19111181
AN - SCOPUS:57749207970
SN - 9780123743787
T3 - Methods in Enzymology
SP - 267
EP - 284
BT - RNA Turnover in Eukaryotes
A2 - Maquat, Lynne
A2 - Kiledjian, Megerditch
ER -