Chapter 1 Protein Phosphorylation by Semisynthesis. From Paper to Practice

Lawrence M. Szewczuk, Mary Katherine Tarrant, Philip A. Cole

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Deconvolution of specific phosphorylation events can be complicated by the reversibility of modification. Protein semisynthesis with phosphonate analogues offers an attractive approach to functional analysis of signaling pathways. In this technique, N- and C-terminal synthetic peptides containing nonhydrolyzable phosphonates at target residues can be ligated to recombinant proteins of interest. The resultant semisynthetic proteins contain site specific, stoichiometric phosphonate modifications and are completely resistant to phosphatases. Control of stoichiometry, specificity, and reversibility allows for complex signaling systems to be broken down into individual events and discretely examined. This chapter outlines the general methods and considerations for designing and carrying out phosphoprotein semisynthetic projects.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
EditorsTom Muir, John Abelson
Pages1-24
Number of pages24
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume462
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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