Chaotic printing of hydrogel carriers for human mesenchymal stem cell expansion

Ryan Hooper; Amanee Abu Arish; Raquel Tejeda Alejandre; Ryan Brune; Ciro Rodriguez; Grissel Trujillo de Santiago; Mario Moisés Alvarez; Kerry Ann Mitchell; David Dean

doi:10.1016/j.procir.2022.06.043

Chaotic printing of hydrogel carriers for human mesenchymal stem cell expansion

Ryan Hooper, Amanee Abu Arish, Raquel Tejeda Alejandre, Ryan Brune, Ciro Rodriguez, Grissel Trujillo de Santiago, Mario Moisés Alvarez, Kerry Ann Mitchell, David Dean

Research output: Contribution to journal › Conference article › peer-review

Abstract

Increasing expansion rate in cell manufacturing systems would allow faster delivery of life-saving treatments to a greater number of patients. Chaotic printing is a novel biofabrication technology that could improve cell expansion capabilities by producing hydrogel filaments layered with open channels, resulting in significantly higher surface area-per-unit-volume of interface between cells and nutrient media compared to existing systems. In this study, we chaotically printed hydrogel filaments laden with clinically-relevant, bone marrow-derived human mesenchymal stem cells. After the first week of a 21-day expansion period, cell-laden hydrogel filaments containing open channels had an optimal expansion rate and 2.1x as many cells as filaments without channels. We then validated a novel bioreactor design for expanding cells under flowing nutrient media conditions.

Original language	English (US)
Pages (from-to)	237-243
Number of pages	7
Journal	Procedia CIRP
Volume	110
Issue number	C
DOIs	https://doi.org/10.1016/j.procir.2022.06.043
State	Published - 2022
Externally published	Yes
Event	5th CIRP Conference on Biomanufacturing, Cirp BioM 2022 - Calabria, Italy Duration: Jun 22 2022 → Jun 24 2022

Keywords

biofabrication
cell expansion
leukemia
mesenchymal stem cell
tissue engineering

ASJC Scopus subject areas

Control and Systems Engineering
Industrial and Manufacturing Engineering

Access to Document

10.1016/j.procir.2022.06.043

Cite this

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title = "Chaotic printing of hydrogel carriers for human mesenchymal stem cell expansion",

abstract = "Increasing expansion rate in cell manufacturing systems would allow faster delivery of life-saving treatments to a greater number of patients. Chaotic printing is a novel biofabrication technology that could improve cell expansion capabilities by producing hydrogel filaments layered with open channels, resulting in significantly higher surface area-per-unit-volume of interface between cells and nutrient media compared to existing systems. In this study, we chaotically printed hydrogel filaments laden with clinically-relevant, bone marrow-derived human mesenchymal stem cells. After the first week of a 21-day expansion period, cell-laden hydrogel filaments containing open channels had an optimal expansion rate and 2.1x as many cells as filaments without channels. We then validated a novel bioreactor design for expanding cells under flowing nutrient media conditions.",

keywords = "biofabrication, cell expansion, leukemia, mesenchymal stem cell, tissue engineering",

author = "Ryan Hooper and Arish, {Amanee Abu} and Alejandre, {Raquel Tejeda} and Ryan Brune and Ciro Rodriguez and {de Santiago}, {Grissel Trujillo} and Alvarez, {Mario Mois{\'e}s} and Mitchell, {Kerry Ann} and David Dean",

note = "Funding Information: We acknowledge and thank the State of Ohio and The Ohio State University, which provided matching funds for an “Accelerator Grant” (GR120515). Special thanks to Diego Alfonso Quevedo Moreno for contributing to the co-axial KSM printhead design, as well as Carlos Fernando Ceballos Gonz{\'a}lez and Edna Johana Bol{\'i}var Monsalve for also contributing to the printhead design and helping us translate protocols to our lab. Schematics created with BioRender.com. Publisher Copyright: {\textcopyright} 2022 The Authors. Published by Elsevier B.V.; 5th CIRP Conference on Biomanufacturing, Cirp BioM 2022 ; Conference date: 22-06-2022 Through 24-06-2022",

year = "2022",

doi = "10.1016/j.procir.2022.06.043",

language = "English (US)",

volume = "110",

pages = "237--243",

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TY - JOUR

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AU - Hooper, Ryan

AU - Arish, Amanee Abu

AU - Alejandre, Raquel Tejeda

AU - Brune, Ryan

AU - Rodriguez, Ciro

AU - de Santiago, Grissel Trujillo

AU - Alvarez, Mario Moisés

AU - Mitchell, Kerry Ann

AU - Dean, David

N1 - Funding Information: We acknowledge and thank the State of Ohio and The Ohio State University, which provided matching funds for an “Accelerator Grant” (GR120515). Special thanks to Diego Alfonso Quevedo Moreno for contributing to the co-axial KSM printhead design, as well as Carlos Fernando Ceballos González and Edna Johana Bolívar Monsalve for also contributing to the printhead design and helping us translate protocols to our lab. Schematics created with BioRender.com. Publisher Copyright: © 2022 The Authors. Published by Elsevier B.V.

PY - 2022

Y1 - 2022

N2 - Increasing expansion rate in cell manufacturing systems would allow faster delivery of life-saving treatments to a greater number of patients. Chaotic printing is a novel biofabrication technology that could improve cell expansion capabilities by producing hydrogel filaments layered with open channels, resulting in significantly higher surface area-per-unit-volume of interface between cells and nutrient media compared to existing systems. In this study, we chaotically printed hydrogel filaments laden with clinically-relevant, bone marrow-derived human mesenchymal stem cells. After the first week of a 21-day expansion period, cell-laden hydrogel filaments containing open channels had an optimal expansion rate and 2.1x as many cells as filaments without channels. We then validated a novel bioreactor design for expanding cells under flowing nutrient media conditions.

AB - Increasing expansion rate in cell manufacturing systems would allow faster delivery of life-saving treatments to a greater number of patients. Chaotic printing is a novel biofabrication technology that could improve cell expansion capabilities by producing hydrogel filaments layered with open channels, resulting in significantly higher surface area-per-unit-volume of interface between cells and nutrient media compared to existing systems. In this study, we chaotically printed hydrogel filaments laden with clinically-relevant, bone marrow-derived human mesenchymal stem cells. After the first week of a 21-day expansion period, cell-laden hydrogel filaments containing open channels had an optimal expansion rate and 2.1x as many cells as filaments without channels. We then validated a novel bioreactor design for expanding cells under flowing nutrient media conditions.

KW - biofabrication

KW - cell expansion

KW - leukemia

KW - mesenchymal stem cell

KW - tissue engineering

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T2 - 5th CIRP Conference on Biomanufacturing, Cirp BioM 2022

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ER -

Chaotic printing of hydrogel carriers for human mesenchymal stem cell expansion

Abstract

Keywords

ASJC Scopus subject areas

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