Chaotic printing of hydrogel carriers for human mesenchymal stem cell expansion

Ryan Hooper, Amanee Abu Arish, Raquel Tejeda Alejandre, Ryan Brune, Ciro Rodriguez, Grissel Trujillo de Santiago, Mario Moisés Alvarez, Kerry Ann Mitchell, David Dean

Research output: Contribution to journalConference articlepeer-review


Increasing expansion rate in cell manufacturing systems would allow faster delivery of life-saving treatments to a greater number of patients. Chaotic printing is a novel biofabrication technology that could improve cell expansion capabilities by producing hydrogel filaments layered with open channels, resulting in significantly higher surface area-per-unit-volume of interface between cells and nutrient media compared to existing systems. In this study, we chaotically printed hydrogel filaments laden with clinically-relevant, bone marrow-derived human mesenchymal stem cells. After the first week of a 21-day expansion period, cell-laden hydrogel filaments containing open channels had an optimal expansion rate and 2.1x as many cells as filaments without channels. We then validated a novel bioreactor design for expanding cells under flowing nutrient media conditions.

Original languageEnglish (US)
Pages (from-to)237-243
Number of pages7
JournalProcedia CIRP
Issue numberC
StatePublished - 2022
Externally publishedYes
Event5th CIRP Conference on Biomanufacturing, Cirp BioM 2022 - Calabria, Italy
Duration: Jun 22 2022Jun 24 2022


  • biofabrication
  • cell expansion
  • leukemia
  • mesenchymal stem cell
  • tissue engineering

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Industrial and Manufacturing Engineering


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