Changes of enzymic activities in human diploid cell line WI-38 at various passages

K. M. Wang, N. R. Rose, E. A. Bartholomew, M. Balzer, K. Berde, M. Foldvary

Research output: Contribution to journalArticle

Abstract

Three groups of enzymes, namely, phosphatases, dehydrogenases and transaminases have been studied in the development of human diploid cells, WI-38, from passage 20 to passage 52. There was a general decrease in the activities of these three groups of enzymes after passage 42 and some increases of enzymic activities in the 31-42 passage range. The total cellular proteins were generally constant from the 20th to the 48th passage and increased after passage 48. However, an increase of protein in a specific cellular fraction at certain passages was observed. From pH and inhibitor studies on WI-38 diploid cells, malate dehydrogenase showed a shift to a high pH optimum at later passages, while acid phosphatase has shown no change to inhibitors from early to later passages. However, sodium tartrate showed an increased inhibitory effect on the soluble Zn2+-activated acid phosphatase as passages progressed. Decreased inhibitory effect by cysteine and cyanide on alkaline phosphatase has been observed in the later passages.

Original languageEnglish (US)
Pages (from-to)357-364
Number of pages8
JournalExperimental Cell Research
Volume61
Issue number2-3
DOIs
StatePublished - 1970
Externally publishedYes

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Acid Phosphatase
Diploidy
Human Activities
Cell Line
Malate Dehydrogenase
Cyanides
Human Development
Enzymes
Transaminases
Phosphoric Monoester Hydrolases
Cysteine
Alkaline Phosphatase
Oxidoreductases
Proteins
tartaric acid

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, K. M., Rose, N. R., Bartholomew, E. A., Balzer, M., Berde, K., & Foldvary, M. (1970). Changes of enzymic activities in human diploid cell line WI-38 at various passages. Experimental Cell Research, 61(2-3), 357-364. https://doi.org/10.1016/0014-4827(70)90458-1

Changes of enzymic activities in human diploid cell line WI-38 at various passages. / Wang, K. M.; Rose, N. R.; Bartholomew, E. A.; Balzer, M.; Berde, K.; Foldvary, M.

In: Experimental Cell Research, Vol. 61, No. 2-3, 1970, p. 357-364.

Research output: Contribution to journalArticle

Wang, KM, Rose, NR, Bartholomew, EA, Balzer, M, Berde, K & Foldvary, M 1970, 'Changes of enzymic activities in human diploid cell line WI-38 at various passages', Experimental Cell Research, vol. 61, no. 2-3, pp. 357-364. https://doi.org/10.1016/0014-4827(70)90458-1
Wang, K. M. ; Rose, N. R. ; Bartholomew, E. A. ; Balzer, M. ; Berde, K. ; Foldvary, M. / Changes of enzymic activities in human diploid cell line WI-38 at various passages. In: Experimental Cell Research. 1970 ; Vol. 61, No. 2-3. pp. 357-364.
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