Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20

Marvin O. Boluyt, Julie L. Brevick, David S. Rogers, Michael J. Randall, Antony F. Scalia, Zhao Bo Li

Research output: Contribution to journalArticle

Abstract

Chronic exercise training elicits adaptations in the heart that improve pump function and confer cardioprotection. To identify molecular mechanisms by which exercise training stimulates this favorable phenotype, a proteomic approach was employed to detect rat cardiac proteins that were differentially expressed or modified after exercise training. Exercise-trained rats underwent six weeks of progressive treadmill training five days/week, 0% grade, using an interval training protocol. Sedentary control rats were age- and weight-matched to the exercise-trained rats. Hearts were harvested at various times (0-72 h) after the last bout of exercise and were used to generate 2-D electrophoretic proteome maps and immunoblots. Compared with hearts of sedentary rats, 26 protein spot intensities were significantly altered in hypertrophied hearts of exercise-trained rats (p

Original languageEnglish (US)
Pages (from-to)3154-3169
Number of pages16
JournalProteomics
Volume6
Issue number10
DOIs
StatePublished - May 2006
Externally publishedYes

Fingerprint

Proteome
Heat-Shock Proteins
Rats
Exercise
Rat control
Exercise equipment
Proteomics
Proteins
Pumps
Phenotype
Weights and Measures

Keywords

  • Cell signaling
  • Differential proteomics
  • Physiology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Boluyt, M. O., Brevick, J. L., Rogers, D. S., Randall, M. J., Scalia, A. F., & Li, Z. B. (2006). Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20. Proteomics, 6(10), 3154-3169. https://doi.org/10.1002/pmic.200401356

Changes in the rat heart proteome induced by exercise training : Increased abundance of heat shock protein hsp20. / Boluyt, Marvin O.; Brevick, Julie L.; Rogers, David S.; Randall, Michael J.; Scalia, Antony F.; Li, Zhao Bo.

In: Proteomics, Vol. 6, No. 10, 05.2006, p. 3154-3169.

Research output: Contribution to journalArticle

Boluyt, MO, Brevick, JL, Rogers, DS, Randall, MJ, Scalia, AF & Li, ZB 2006, 'Changes in the rat heart proteome induced by exercise training: Increased abundance of heat shock protein hsp20', Proteomics, vol. 6, no. 10, pp. 3154-3169. https://doi.org/10.1002/pmic.200401356
Boluyt, Marvin O. ; Brevick, Julie L. ; Rogers, David S. ; Randall, Michael J. ; Scalia, Antony F. ; Li, Zhao Bo. / Changes in the rat heart proteome induced by exercise training : Increased abundance of heat shock protein hsp20. In: Proteomics. 2006 ; Vol. 6, No. 10. pp. 3154-3169.
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