Changes in the organization of the sea urchin egg plasma membrane upon fertilization: Indications from the lateral diffusion rates of lipid-soluble fluorescent dyes

David E. Wolf, William Kinsey, William Lennarz, Michael Edidin

Research output: Contribution to journalArticlepeer-review

Abstract

We have used fluorescence photobleaching recovery to measure the diffusion of lipid probes (1,2-acyl-2-(N-4-nitro-benzo-2-oxo-1,3-diazole)aminocaproylphosphatidylcholine (NBD-PC)) and the 3,3′-diacylindocarbocyanine iodides (CndiI) with even acyl chain lengths (n = 10 to 18) in the plasma membranes of the sea urchin egg Strongylocentrotus purpuratus before and after fertilization. At 15°C measured diffusion coefficients, D, ranged from (1 to 5) × 10-9 cm2/sec, diffusing fractions from 36 to 77% for the CndI's. D shows a relative minimum at n = 12 for unfertilized eggs, a relative maximum at n = 14 for fertilized eggs. Thus the effect of fertilization on diffusion coefficient is dependent on probe structure. The fraction of molecules free to diffuse also depends upon acyl chain length; this fraction was smaller in fertilized eggs than in unfertilized eggs for all chain lengths. Our results indicate that different lipid analogs probe different microenvironments and suggest the existence of domains of lipids differing in composition or physical state from the average for the egg plasma membrane.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
JournalDevelopmental biology
Volume81
Issue number1
DOIs
StatePublished - Jan 15 1981

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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