TY - JOUR
T1 - Changes in the oligodendrocyte progenitor cell proteome with ageing
AU - de la Fuente, Alerie G.
AU - Queiroz, Rayner M.L.
AU - Ghosh, Tanay
AU - McMurran, Christopher E.
AU - Cubillos, Juan F.
AU - Bergles, Dwight E.
AU - Fitzgerald, Denise C.
AU - Jones, Clare A.
AU - Lilley, Kathryn S.
AU - Glover, Colin P.
AU - Franklin, Robin J.M.
N1 - Funding Information:
Adelson Medical Research Foundation and a core support grant from the Wellcome Trust and MRC to
Funding Information:
This work was supported by grants from the UK Multiple Sclerosis Society (MS50), MedImmune, The Adelson Medical Research Foundation and a core support grant from the Wellcome Trust and MRC to the Wellcome-Medical Research Council Cambridge Stem Cell Institute (203151/Z/16/Z). AGF was also supported by an ECTRIMS postdoctoral fellowship from July 2018. RQ was supported by the Brazilian Science without Borders program (grant number 203098/2014-5 - AstraZeneca-Brasil/CNPq). The authors have no conflicting financial interests. We also thank Sonja Hess, Raghothama Chaerkady, Gina D?Angelo and Wen Yu from AstraZeneca for running the mass spectrometers and especially Raghothama Chaerkady for also helping drawing the graphical abstract.
Funding Information:
supported by an ECTRIMS postdoctoral fellowship from July 2018. RQ was supported by the Brazilian
Funding Information:
This work was supported by grants from the UK Multiple Sclerosis Society (MS50), MedImmune, The
Publisher Copyright:
© 2020 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - Following central nervous system (CNS) demyelination, adult oligodendrocyte progenitor cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative process called remyelination. While remyelination is very efficient in young adults, its efficiency declines progressively with ageing. Here we performed proteomic analysis of OPCs freshly isolated from the brains of neonate, young and aged female rats. Approximately 50% of the proteins are expressed at different levels in OPCs from neonates compared to their adult counterparts. The amount of myelin-associated proteins, and proteins associated with oxidative phosphorylation, inflammatory responses and actin cytoskeletal organization increased with age, while cholesterol-biosynthesis, transcription factors and cell cycle proteins decreased. Our experiments provide the first ageing OPC proteome, revealing the distinct features of OPCs at different ages. These studies provide new insights into why remyelination efficiency declines with ageing and potential roles for aged OPCs in other neurodegenerative diseases.
AB - Following central nervous system (CNS) demyelination, adult oligodendrocyte progenitor cells (OPCs) can differentiate into new myelin-forming oligodendrocytes in a regenerative process called remyelination. While remyelination is very efficient in young adults, its efficiency declines progressively with ageing. Here we performed proteomic analysis of OPCs freshly isolated from the brains of neonate, young and aged female rats. Approximately 50% of the proteins are expressed at different levels in OPCs from neonates compared to their adult counterparts. The amount of myelin-associated proteins, and proteins associated with oxidative phosphorylation, inflammatory responses and actin cytoskeletal organization increased with age, while cholesterol-biosynthesis, transcription factors and cell cycle proteins decreased. Our experiments provide the first ageing OPC proteome, revealing the distinct features of OPCs at different ages. These studies provide new insights into why remyelination efficiency declines with ageing and potential roles for aged OPCs in other neurodegenerative diseases.
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U2 - 10.1074/mcp.RA120.002102
DO - 10.1074/mcp.RA120.002102
M3 - Article
C2 - 32434922
AN - SCOPUS:85089127678
SN - 1535-9476
VL - 19
SP - 1281
EP - 1302
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 8
ER -