Changes in scleral collagen organization in murine chronic experimental glaucoma

Jacek K. Pijanka, Elizabeth C. Kimball, Mary E. Pease, Ahmed Abass, Thomas Sorensen, Thao D. Nguyen, Harry A. Quigley, Craig Boote

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE: The organization of scleral collagen helps to determine the eye's biomechanical response to intraocular pressure (IOP), and may therefore be important in glaucoma. This study provides a quantitative assessment of changes in scleral collagen fibril organization in bead-induced murine experimental glaucoma.

METHODS: Wide-angle X-ray scattering was used to study the effect of bead-induced glaucoma on posterior scleral collagen organization in one eye of 12 CD1 mice, with untreated fellow eyes serving as controls. Three collagen parameters were measured: the local preferred fibril directions, the degree of collagen anisotropy, and the total fibrillar collagen content.

RESULTS: The mouse sclera featured a largely circumferential orientation of fibrillar collagen with respect to the optic nerve head canal. Localized alteration to fibril orientations was evident in the inferior peripapillary sclera of bead-treated eyes. Collagen anisotropy was significantly (P<0.05) reduced in bead-treated eyes in the superior peripapillary (Treated: 43±8%;

CONTROL: 49±6%) and midposterior (Treated: 39±4%;

CONTROL: 43±4%) sclera, and in the peripapillary region overall (Treated: 43±6%;

CONTROL: 47±3%). No significant differences in total collagen content were found between groups.

CONCLUSIONS: Spatial changes in collagen fibril anisotropy occur in the posterior sclera of mice with bead-induced chronic IOP elevation and axonal damage. These results support the idea that dynamic changes in scleral form and structure play a role in the development of experimental glaucoma in mice, and potentially in human glaucoma.

Original languageEnglish (US)
Pages (from-to)6554-6564
Number of pages11
JournalInvestigative ophthalmology & visual science
Volume55
Issue number10
DOIs
StatePublished - 2014
Externally publishedYes

Keywords

  • X-ray scattering
  • collagen
  • glaucoma
  • mouse
  • sclera

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Changes in scleral collagen organization in murine chronic experimental glaucoma'. Together they form a unique fingerprint.

Cite this