Processing of rRNA was examined in resting and phytohemagglutinin stimulated guinea pig lymphocytes. Synthesis of 1.7 (28S) and 0.7 (18S) X 106 dalton rRNA was more than 4 fold greater in phytohemagglutinin stimulated than in resting cells. A 5 to 10 fold increase in flux of molecules through a 2.3 x 106 dalton RNA occurred without a concurrent change in the flux through a 2.6 x 106 dalton fraction in phytohemagglutinin stimulated cells. In both resting and phytohemagglutinin stimulated lymphocytes, the 2.3 x 106 dalton intermediate equilibrated with [3H]methyl label and pulse chased prior to the 2.6 x 106 dalton RNA. The data indicate at least two processing paths in guinea pig lymphocytes; one proceeds to rRNA via a 2.3 x 106 dalton intermediate, and another proceeds via a 2.6 x 106 dalton RNA. The increase in rRNA synthesis in phytohemagglutinin stimulated cells occurs primarily through that path containing the 2.3 x 106 dalton intermediate.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - 1975|
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