TY - JOUR
T1 - Changes in rate of RNA synthesis and ribosomal gene number during oogenesis of Drosophila melanogaster
AU - Mermod, Jean Jacques
AU - Jacobs-Lorena, Marcelo
AU - Crippa, Marco
N1 - Funding Information:
The authors wish to thank Mrs. Noelle Charpen-tier for her excellent technical assistance, and are grateful to Professor Leonard Nelson for reading the manuscript. This investigation was supported by Grant No. 3.318.74 from the Swiss National Fund.
PY - 1977/6
Y1 - 1977/6
N2 - A new method for separating Drosophila egg chambers into different developmental classes (Jacobs-Lorena and Crippa, 1977) made it possible to study changes in the rate of ribosomal RNA (rRNA), 5S RNA, and tRNA synthesis and the changes in ribosomal gene number during oogenesis. Synthesis of RNA was measured by [3H]uridine incorporation in vivo and subsequent analysis on sucrose gradients or gel electrophoresis. Specific radioactivity of nucleotide pools has also been determined. Ribosomal gene number has been measured by hybridization of egg chamber DNA to rRNA of high specific radioactivity. Our findings led us to conclude that in Drosophila melanogaster: (i) rRNA, 5S RNA, and tRNA are synthesized in all stages of oogenesis. (ii) In every stage, rRNA is the main RNA species synthesized. (iii) The rate of rRNA, 5S RNA, and tRNA synthesis increases greatly during oogenesis and is paralleled by a similar increase in ribosomal gene number resulting from the polyploidization of the nurse cell nuclei.
AB - A new method for separating Drosophila egg chambers into different developmental classes (Jacobs-Lorena and Crippa, 1977) made it possible to study changes in the rate of ribosomal RNA (rRNA), 5S RNA, and tRNA synthesis and the changes in ribosomal gene number during oogenesis. Synthesis of RNA was measured by [3H]uridine incorporation in vivo and subsequent analysis on sucrose gradients or gel electrophoresis. Specific radioactivity of nucleotide pools has also been determined. Ribosomal gene number has been measured by hybridization of egg chamber DNA to rRNA of high specific radioactivity. Our findings led us to conclude that in Drosophila melanogaster: (i) rRNA, 5S RNA, and tRNA are synthesized in all stages of oogenesis. (ii) In every stage, rRNA is the main RNA species synthesized. (iii) The rate of rRNA, 5S RNA, and tRNA synthesis increases greatly during oogenesis and is paralleled by a similar increase in ribosomal gene number resulting from the polyploidization of the nurse cell nuclei.
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U2 - 10.1016/0012-1606(77)90224-X
DO - 10.1016/0012-1606(77)90224-X
M3 - Article
C2 - 406151
AN - SCOPUS:0017377902
SN - 0012-1606
VL - 57
SP - 393
EP - 402
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -