Changes in neurofilament and microtubule distribution following focal axon compression

Adam J. Fournier, James D. Hogan, Labchan Rajbhandari, Shiva Shrestha, Arun Venkatesan, K. T. Ramesh

Research output: Contribution to journalArticle

Abstract

Although a number of cytoskeletal derangements have been described in the setting of traumatic axonal injury (TAI), little is known of early structural changes that may serve to initiate a cascade of further axonal degeneration. Recent work by the authors has examined conformational changes in cytoskeletal constituents of neuronal axons undergoing traumatic axonal injury (TAI) following focal compression through confocal imaging data taken in vitro and in situ. The present study uses electron microscopy to understand and quantify in vitro alterations in the ultrastructural composition of microtubules and neurofilaments within neuronal axons of rats following focal compression. Standard transmission electron microscopy processing methods are used to identify microtubules, while neurofilament identification is performed using antibody labeling through gold nanoparticles. The number, density, and spacing of microtubules and neurofilaments are quantified for specimens in sham Control and Crushed groups with fixation at

Original languageEnglish (US)
Article numbere0131617
JournalPLoS One
Volume10
Issue number6
DOIs
StatePublished - Jun 25 2015

Fingerprint

Intermediate Filaments
axons
Microtubules
microtubules
Axons
Gold
Labeling
Electron microscopy
Rats
Wounds and Injuries
Nanoparticles
Transmission electron microscopy
processing technology
Transmission Electron Microscopy
Imaging techniques
transmission electron microscopy
Antibodies
electron microscopy
Electron Microscopy
Processing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fournier, A. J., Hogan, J. D., Rajbhandari, L., Shrestha, S., Venkatesan, A., & Ramesh, K. T. (2015). Changes in neurofilament and microtubule distribution following focal axon compression. PLoS One, 10(6), [e0131617]. https://doi.org/10.1371/journal.pone.0131617

Changes in neurofilament and microtubule distribution following focal axon compression. / Fournier, Adam J.; Hogan, James D.; Rajbhandari, Labchan; Shrestha, Shiva; Venkatesan, Arun; Ramesh, K. T.

In: PLoS One, Vol. 10, No. 6, e0131617, 25.06.2015.

Research output: Contribution to journalArticle

Fournier, AJ, Hogan, JD, Rajbhandari, L, Shrestha, S, Venkatesan, A & Ramesh, KT 2015, 'Changes in neurofilament and microtubule distribution following focal axon compression', PLoS One, vol. 10, no. 6, e0131617. https://doi.org/10.1371/journal.pone.0131617
Fournier, Adam J. ; Hogan, James D. ; Rajbhandari, Labchan ; Shrestha, Shiva ; Venkatesan, Arun ; Ramesh, K. T. / Changes in neurofilament and microtubule distribution following focal axon compression. In: PLoS One. 2015 ; Vol. 10, No. 6.
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