Abstract
Although a number of cytoskeletal derangements have been described in the setting of traumatic axonal injury (TAI), little is known of early structural changes that may serve to initiate a cascade of further axonal degeneration. Recent work by the authors has examined conformational changes in cytoskeletal constituents of neuronal axons undergoing traumatic axonal injury (TAI) following focal compression through confocal imaging data taken in vitro and in situ. The present study uses electron microscopy to understand and quantify in vitro alterations in the ultrastructural composition of microtubules and neurofilaments within neuronal axons of rats following focal compression. Standard transmission electron microscopy processing methods are used to identify microtubules, while neurofilament identification is performed using antibody labeling through gold nanoparticles. The number, density, and spacing of microtubules and neurofilaments are quantified for specimens in sham Control and Crushed groups with fixation at
| Original language | English (US) |
|---|---|
| Article number | e0131617 |
| Journal | PLoS One |
| Volume | 10 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 25 2015 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Changes in neurofilament and microtubule distribution following focal axon compression. / Fournier, Adam J.; Hogan, James D.; Rajbhandari, Labchan; Shrestha, Shiva; Venkatesan, Arun; Ramesh, K. T.
In: PLoS One, Vol. 10, No. 6, e0131617, 25.06.2015.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Changes in neurofilament and microtubule distribution following focal axon compression
AU - Fournier, Adam J.
AU - Hogan, James D.
AU - Rajbhandari, Labchan
AU - Shrestha, Shiva
AU - Venkatesan, Arun
AU - Ramesh, K. T.
PY - 2015/6/25
Y1 - 2015/6/25
N2 - Although a number of cytoskeletal derangements have been described in the setting of traumatic axonal injury (TAI), little is known of early structural changes that may serve to initiate a cascade of further axonal degeneration. Recent work by the authors has examined conformational changes in cytoskeletal constituents of neuronal axons undergoing traumatic axonal injury (TAI) following focal compression through confocal imaging data taken in vitro and in situ. The present study uses electron microscopy to understand and quantify in vitro alterations in the ultrastructural composition of microtubules and neurofilaments within neuronal axons of rats following focal compression. Standard transmission electron microscopy processing methods are used to identify microtubules, while neurofilament identification is performed using antibody labeling through gold nanoparticles. The number, density, and spacing of microtubules and neurofilaments are quantified for specimens in sham Control and Crushed groups with fixation at
AB - Although a number of cytoskeletal derangements have been described in the setting of traumatic axonal injury (TAI), little is known of early structural changes that may serve to initiate a cascade of further axonal degeneration. Recent work by the authors has examined conformational changes in cytoskeletal constituents of neuronal axons undergoing traumatic axonal injury (TAI) following focal compression through confocal imaging data taken in vitro and in situ. The present study uses electron microscopy to understand and quantify in vitro alterations in the ultrastructural composition of microtubules and neurofilaments within neuronal axons of rats following focal compression. Standard transmission electron microscopy processing methods are used to identify microtubules, while neurofilament identification is performed using antibody labeling through gold nanoparticles. The number, density, and spacing of microtubules and neurofilaments are quantified for specimens in sham Control and Crushed groups with fixation at
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U2 - 10.1371/journal.pone.0131617
DO - 10.1371/journal.pone.0131617
M3 - Article
C2 - 26111004
AN - SCOPUS:84938630423
VL - 10
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 6
M1 - e0131617
ER -