Changes in lens crystalling during cataract development in the philly mouse

J. S. Zigler, D. A. Carper, J. H. Kinoshita

Research output: Contribution to journalArticlepeer-review

Abstract

Laurell ‘rocket’ immunoelectrophoresis has been applied to the quantitation of lens crystallins during development of hereditary cataract in the Philly mouse. A marked decrease in γ crystallin and smaller loss of β crystallin was found to accompany catarac-togenesis. Both β and γ crystallins were identified in the aqueous humor of the Philly mouse following cataract development. The Philly mouse lens was shown to lack one β crystallin antigen seen in the normal mouse by the rocket technique. This observation was correlated with altered composition of β crystallin polypeptides and oligomers. The rocket method was also applied to quantitation of human α, β, and γ crystallin.

Original languageEnglish (US)
Pages (from-to)237-251
Number of pages15
JournalOphthalmic Research
Volume13
Issue number5
DOIs
StatePublished - 1981

Keywords

  • Cataract
  • Crystallin quantitation
  • Degradation
  • Insolubilization
  • Leakage
  • Lens crystallins
  • Philly mouse
  • Rocket immunoelectrophoresis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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