Changes in Gut Microbiome after Bariatric Surgery Versus Medical Weight Loss in a Pilot Randomized Trial

Research output: Contribution to journalArticle

Abstract

Background: Gut microbiota likely impact obesity and metabolic diseases. We evaluated the changes in gut microbiota after surgical versus medical weight loss in adults with diabetes and obesity. Methods: We performed 16S rRNA amplicon sequencing to identify the gut microbial composition at baseline and at 10% weight loss in adults with diabetes who were randomized to medical weight loss (MWL, n = 4), adjustable gastric banding (AGB, n = 4), or Roux-en-Y gastric bypass (RYGB, n = 4). Results: All participants were female, 75% reported black race with mean age of 51 years. At similar weight loss amount and glycemic improvement, the RYGB group had the most number of bacterial species (10 increased, 1 decreased) that significantly changed (p < 0.05) in relative abundance. Alpha-diversity at follow-up was significantly lower in AGB group compared to MWL and RYGB (observed species for AGB vs. MWL, p = 0.0093; AGB vs. RYGB, p = 0.0093). The relative abundance of Faecalibacterium prausnitzii increased in 3 participants after RYGB, 1 after AGB, and 1 after MWL. Conclusions: At similar weight loss and glycemic improvement, the greatest alteration in gut microbiota occurred after RYGB with an increase in the potentially beneficial bacterium, F. prausnitzii. Gut microbial diversity tended to decrease after AGB and increase after RYGB and MWL. Future studies are needed to determine the impact and durability of gut microbial changes over time and their role in long-term metabolic improvement after bariatric surgery in adults with type 2 diabetes. Clinical Trial Registration: NCTDK089557—ClinicalTrials.gov.

Original languageEnglish (US)
JournalObesity Surgery
DOIs
StatePublished - Jan 1 2019

Fingerprint

Bariatric Surgery
Weight Loss
Obesity
Gastric Bypass
Metabolic Diseases
Type 2 Diabetes Mellitus
Stomach
Gastrointestinal Microbiome
Clinical Trials
Bacteria

Keywords

  • Diabetes
  • Gastric band
  • Gastric bypass
  • Gut microbiome
  • Obesity
  • Randomized controlled trial

ASJC Scopus subject areas

  • Surgery
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

@article{ba69f9793a8e403dbda73f96d75c37a2,
title = "Changes in Gut Microbiome after Bariatric Surgery Versus Medical Weight Loss in a Pilot Randomized Trial",
abstract = "Background: Gut microbiota likely impact obesity and metabolic diseases. We evaluated the changes in gut microbiota after surgical versus medical weight loss in adults with diabetes and obesity. Methods: We performed 16S rRNA amplicon sequencing to identify the gut microbial composition at baseline and at 10{\%} weight loss in adults with diabetes who were randomized to medical weight loss (MWL, n = 4), adjustable gastric banding (AGB, n = 4), or Roux-en-Y gastric bypass (RYGB, n = 4). Results: All participants were female, 75{\%} reported black race with mean age of 51 years. At similar weight loss amount and glycemic improvement, the RYGB group had the most number of bacterial species (10 increased, 1 decreased) that significantly changed (p < 0.05) in relative abundance. Alpha-diversity at follow-up was significantly lower in AGB group compared to MWL and RYGB (observed species for AGB vs. MWL, p = 0.0093; AGB vs. RYGB, p = 0.0093). The relative abundance of Faecalibacterium prausnitzii increased in 3 participants after RYGB, 1 after AGB, and 1 after MWL. Conclusions: At similar weight loss and glycemic improvement, the greatest alteration in gut microbiota occurred after RYGB with an increase in the potentially beneficial bacterium, F. prausnitzii. Gut microbial diversity tended to decrease after AGB and increase after RYGB and MWL. Future studies are needed to determine the impact and durability of gut microbial changes over time and their role in long-term metabolic improvement after bariatric surgery in adults with type 2 diabetes. Clinical Trial Registration: NCTDK089557—ClinicalTrials.gov.",
keywords = "Diabetes, Gastric band, Gastric bypass, Gut microbiome, Obesity, Randomized controlled trial",
author = "Clare Lee and Florea, {Liliana D} and Sears, {Cynthia Louise} and Nisa Maruthur and Potter, {James John} and Schweitzer, {Michael A} and Thomas Magnuson and Jeanne Clark",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s11695-019-03976-4",
language = "English (US)",
journal = "Obesity Surgery",
issn = "0960-8923",
publisher = "Springer New York",

}

TY - JOUR

T1 - Changes in Gut Microbiome after Bariatric Surgery Versus Medical Weight Loss in a Pilot Randomized Trial

AU - Lee, Clare

AU - Florea, Liliana D

AU - Sears, Cynthia Louise

AU - Maruthur, Nisa

AU - Potter, James John

AU - Schweitzer, Michael A

AU - Magnuson, Thomas

AU - Clark, Jeanne

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: Gut microbiota likely impact obesity and metabolic diseases. We evaluated the changes in gut microbiota after surgical versus medical weight loss in adults with diabetes and obesity. Methods: We performed 16S rRNA amplicon sequencing to identify the gut microbial composition at baseline and at 10% weight loss in adults with diabetes who were randomized to medical weight loss (MWL, n = 4), adjustable gastric banding (AGB, n = 4), or Roux-en-Y gastric bypass (RYGB, n = 4). Results: All participants were female, 75% reported black race with mean age of 51 years. At similar weight loss amount and glycemic improvement, the RYGB group had the most number of bacterial species (10 increased, 1 decreased) that significantly changed (p < 0.05) in relative abundance. Alpha-diversity at follow-up was significantly lower in AGB group compared to MWL and RYGB (observed species for AGB vs. MWL, p = 0.0093; AGB vs. RYGB, p = 0.0093). The relative abundance of Faecalibacterium prausnitzii increased in 3 participants after RYGB, 1 after AGB, and 1 after MWL. Conclusions: At similar weight loss and glycemic improvement, the greatest alteration in gut microbiota occurred after RYGB with an increase in the potentially beneficial bacterium, F. prausnitzii. Gut microbial diversity tended to decrease after AGB and increase after RYGB and MWL. Future studies are needed to determine the impact and durability of gut microbial changes over time and their role in long-term metabolic improvement after bariatric surgery in adults with type 2 diabetes. Clinical Trial Registration: NCTDK089557—ClinicalTrials.gov.

AB - Background: Gut microbiota likely impact obesity and metabolic diseases. We evaluated the changes in gut microbiota after surgical versus medical weight loss in adults with diabetes and obesity. Methods: We performed 16S rRNA amplicon sequencing to identify the gut microbial composition at baseline and at 10% weight loss in adults with diabetes who were randomized to medical weight loss (MWL, n = 4), adjustable gastric banding (AGB, n = 4), or Roux-en-Y gastric bypass (RYGB, n = 4). Results: All participants were female, 75% reported black race with mean age of 51 years. At similar weight loss amount and glycemic improvement, the RYGB group had the most number of bacterial species (10 increased, 1 decreased) that significantly changed (p < 0.05) in relative abundance. Alpha-diversity at follow-up was significantly lower in AGB group compared to MWL and RYGB (observed species for AGB vs. MWL, p = 0.0093; AGB vs. RYGB, p = 0.0093). The relative abundance of Faecalibacterium prausnitzii increased in 3 participants after RYGB, 1 after AGB, and 1 after MWL. Conclusions: At similar weight loss and glycemic improvement, the greatest alteration in gut microbiota occurred after RYGB with an increase in the potentially beneficial bacterium, F. prausnitzii. Gut microbial diversity tended to decrease after AGB and increase after RYGB and MWL. Future studies are needed to determine the impact and durability of gut microbial changes over time and their role in long-term metabolic improvement after bariatric surgery in adults with type 2 diabetes. Clinical Trial Registration: NCTDK089557—ClinicalTrials.gov.

KW - Diabetes

KW - Gastric band

KW - Gastric bypass

KW - Gut microbiome

KW - Obesity

KW - Randomized controlled trial

UR - http://www.scopus.com/inward/record.url?scp=85068345690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068345690&partnerID=8YFLogxK

U2 - 10.1007/s11695-019-03976-4

DO - 10.1007/s11695-019-03976-4

M3 - Article

C2 - 31256356

AN - SCOPUS:85068345690

JO - Obesity Surgery

JF - Obesity Surgery

SN - 0960-8923

ER -