Changes in genome organization of parasite-specific gene families during the Plasmodium transmission stages

Evelien M. Bunnik, Kate B. Cook, Nelle Varoquaux, Gayani Batugedara, Jacques Prudhomme, Anthony Cort, Lirong Shi, Chiara Andolina, Leila S. Ross, Declan Brady, David A. Fidock, Francois Nosten, Rita Tewari, Photini Sinnis, Ferhat Ay, Jean Philippe Vert, William Stafford Noble, Karine G. Le Roch

Research output: Contribution to journalArticle

Abstract

The development of malaria parasites throughout their various life cycle stages is coordinated by changes in gene expression. We previously showed that the three-dimensional organization of the Plasmodium falciparum genome is strongly associated with gene expression during its replication cycle inside red blood cells. Here, we analyze genome organization in the P. falciparum and P. vivax transmission stages. Major changes occur in the localization and interactions of genes involved in pathogenesis and immune evasion, host cell invasion, sexual differentiation, and master regulation of gene expression. Furthermore, we observe reorganization of subtelomeric heterochromatin around genes involved in host cell remodeling. Depletion of heterochromatin protein 1 (PfHP1) resulted in loss of interactions between virulence genes, confirming that PfHP1 is essential for maintenance of the repressive center. Our results suggest that the three-dimensional genome structure of human malaria parasites is strongly connected with transcriptional activity of specific gene families throughout the life cycle.

Original languageEnglish (US)
Article number1910
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

parasites
Plasmodium
genome
genes
gene expression
Parasites
Genes
Genome
Plasmodium falciparum
cycles
Malaria
Gene expression
virulence
Immune Evasion
Gene Expression
pathogenesis
Sex Differentiation
Heterochromatin
Gene Expression Regulation
erythrocytes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Bunnik, E. M., Cook, K. B., Varoquaux, N., Batugedara, G., Prudhomme, J., Cort, A., ... Le Roch, K. G. (2018). Changes in genome organization of parasite-specific gene families during the Plasmodium transmission stages. Nature Communications, 9(1), [1910]. https://doi.org/10.1038/s41467-018-04295-5

Changes in genome organization of parasite-specific gene families during the Plasmodium transmission stages. / Bunnik, Evelien M.; Cook, Kate B.; Varoquaux, Nelle; Batugedara, Gayani; Prudhomme, Jacques; Cort, Anthony; Shi, Lirong; Andolina, Chiara; Ross, Leila S.; Brady, Declan; Fidock, David A.; Nosten, Francois; Tewari, Rita; Sinnis, Photini; Ay, Ferhat; Vert, Jean Philippe; Noble, William Stafford; Le Roch, Karine G.

In: Nature Communications, Vol. 9, No. 1, 1910, 01.12.2018.

Research output: Contribution to journalArticle

Bunnik, EM, Cook, KB, Varoquaux, N, Batugedara, G, Prudhomme, J, Cort, A, Shi, L, Andolina, C, Ross, LS, Brady, D, Fidock, DA, Nosten, F, Tewari, R, Sinnis, P, Ay, F, Vert, JP, Noble, WS & Le Roch, KG 2018, 'Changes in genome organization of parasite-specific gene families during the Plasmodium transmission stages', Nature Communications, vol. 9, no. 1, 1910. https://doi.org/10.1038/s41467-018-04295-5
Bunnik, Evelien M. ; Cook, Kate B. ; Varoquaux, Nelle ; Batugedara, Gayani ; Prudhomme, Jacques ; Cort, Anthony ; Shi, Lirong ; Andolina, Chiara ; Ross, Leila S. ; Brady, Declan ; Fidock, David A. ; Nosten, Francois ; Tewari, Rita ; Sinnis, Photini ; Ay, Ferhat ; Vert, Jean Philippe ; Noble, William Stafford ; Le Roch, Karine G. / Changes in genome organization of parasite-specific gene families during the Plasmodium transmission stages. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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