TY - JOUR
T1 - Changes in expression of the 44-kilodalton outer surface membrane antigen (p44 kD) for monitoring progression of infection and antimicrobial susceptibility of the human granulocytic ehrlichiosis (HGE) agent in HL-60 cells
AU - Hsieh, Tze Chen
AU - Dipietrantonio, Anna M.
AU - Horowitz, Harold W.
AU - Dumler, J. Stephen
AU - Aguero-Rosenfeld, Maria E.
AU - Wormser, Gary P.
AU - Wu, Joseph M.
N1 - Funding Information:
This research was supported by the Vivian Wu-Au Cancer Memorial Fund and an unrestricted grant from the Philip Morris Co. (to J.M.W.); the Westchester County Department of Health (Grant CMC-2502 to H.W.H. and Grants HLT27017, HLT27018, and HLT27019 to M.E.A.); and the National Institute of Health (Grant R01-AI41213 to S.I.D.).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1999/4/13
Y1 - 1999/4/13
N2 - Changes in human granulocytic ehrlichiosis (HGE)-specific major outer membrane protein (p44 kD) were assayed by Western blot analysis in HL-60 cells in vitro infected by the HGE agent. Time course study demonstrated that the expression of p44 preceded the rise in cell infection as determined by the presence of intracellular morulae. To test whether the expression of p44 may be suitable for evaluating the effects of antibiotics in vitro, three recent isolates of the HGE agent were exposed to doxycycline and ampicillin during culture with HL-60 cells. Loss of infection concurrent with disappearance of the 44 kD protein was found with doxycycline treatment. In contrast, ampicillin treatment had no discernible effects on infection or 44 kD expression. There was excellent agreement between infection, as measured by morulae, and 44 kD) expression (coefficient of correlation r = 0.97, p < 0.01). Following treatment with doxycycline, the 44 kD protein disappeared with an estimated t(1/2) of ~24-30 h, which was considerably shorter than a t(1/2) of > 60 h calculated for loss of morulae. Measurement of p44 expression may be a more rapid and simple assay to determine antibiotic susceptibility of the HGE agent in cell culture. Furthermore, it may be used to indicate the presence of infection before morulae are apparent.
AB - Changes in human granulocytic ehrlichiosis (HGE)-specific major outer membrane protein (p44 kD) were assayed by Western blot analysis in HL-60 cells in vitro infected by the HGE agent. Time course study demonstrated that the expression of p44 preceded the rise in cell infection as determined by the presence of intracellular morulae. To test whether the expression of p44 may be suitable for evaluating the effects of antibiotics in vitro, three recent isolates of the HGE agent were exposed to doxycycline and ampicillin during culture with HL-60 cells. Loss of infection concurrent with disappearance of the 44 kD protein was found with doxycycline treatment. In contrast, ampicillin treatment had no discernible effects on infection or 44 kD expression. There was excellent agreement between infection, as measured by morulae, and 44 kD) expression (coefficient of correlation r = 0.97, p < 0.01). Following treatment with doxycycline, the 44 kD protein disappeared with an estimated t(1/2) of ~24-30 h, which was considerably shorter than a t(1/2) of > 60 h calculated for loss of morulae. Measurement of p44 expression may be a more rapid and simple assay to determine antibiotic susceptibility of the HGE agent in cell culture. Furthermore, it may be used to indicate the presence of infection before morulae are apparent.
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U2 - 10.1006/bbrc.1999.0457
DO - 10.1006/bbrc.1999.0457
M3 - Article
C2 - 10198216
AN - SCOPUS:0033551175
SN - 0006-291X
VL - 257
SP - 351
EP - 355
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -