Changes in expression of the 44-kilodalton outer surface membrane antigen (p44 kD) for monitoring progression of infection and antimicrobial susceptibility of the human granulocytic ehrlichiosis (HGE) agent in HL-60 cells

Tze Chen Hsieh, Anna M. Dipietrantonio, Harold W. Horowitz, J. Stephen Dumler, Maria E. Aguero-Rosenfeld, Gary P. Wormser, Joseph M. Wu

Research output: Contribution to journalArticle

Abstract

Changes in human granulocytic ehrlichiosis (HGE)-specific major outer membrane protein (p44 kD) were assayed by Western blot analysis in HL-60 cells in vitro infected by the HGE agent. Time course study demonstrated that the expression of p44 preceded the rise in cell infection as determined by the presence of intracellular morulae. To test whether the expression of p44 may be suitable for evaluating the effects of antibiotics in vitro, three recent isolates of the HGE agent were exposed to doxycycline and ampicillin during culture with HL-60 cells. Loss of infection concurrent with disappearance of the 44 kD protein was found with doxycycline treatment. In contrast, ampicillin treatment had no discernible effects on infection or 44 kD expression. There was excellent agreement between infection, as measured by morulae, and 44 kD) expression (coefficient of correlation r = 0.97, p <0.01). Following treatment with doxycycline, the 44 kD protein disappeared with an estimated t(1/2) of ~24-30 h, which was considerably shorter than a t(1/2) of > 60 h calculated for loss of morulae. Measurement of p44 expression may be a more rapid and simple assay to determine antibiotic susceptibility of the HGE agent in cell culture. Furthermore, it may be used to indicate the presence of infection before morulae are apparent.

Original languageEnglish (US)
Pages (from-to)351-355
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume257
Issue number2
DOIs
StatePublished - Apr 13 1999
Externally publishedYes

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Ehrlichiosis
HL-60 Cells
Surface Antigens
Morula
Doxycycline
Ampicillin
Membranes
Antigens
Monitoring
Infection
Anti-Bacterial Agents
Time and motion study
Cell culture
Assays
Membrane Proteins
Cell Culture Techniques
Western Blotting
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Changes in expression of the 44-kilodalton outer surface membrane antigen (p44 kD) for monitoring progression of infection and antimicrobial susceptibility of the human granulocytic ehrlichiosis (HGE) agent in HL-60 cells. / Hsieh, Tze Chen; Dipietrantonio, Anna M.; Horowitz, Harold W.; Dumler, J. Stephen; Aguero-Rosenfeld, Maria E.; Wormser, Gary P.; Wu, Joseph M.

In: Biochemical and Biophysical Research Communications, Vol. 257, No. 2, 13.04.1999, p. 351-355.

Research output: Contribution to journalArticle

Hsieh, Tze Chen ; Dipietrantonio, Anna M. ; Horowitz, Harold W. ; Dumler, J. Stephen ; Aguero-Rosenfeld, Maria E. ; Wormser, Gary P. ; Wu, Joseph M. / Changes in expression of the 44-kilodalton outer surface membrane antigen (p44 kD) for monitoring progression of infection and antimicrobial susceptibility of the human granulocytic ehrlichiosis (HGE) agent in HL-60 cells. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 257, No. 2. pp. 351-355.
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