Changes in AMPA receptor phosphorylation in the rostral ventromedial medulla after inflammatory hyperalgesia in rats

Yun Guan, Wei Guo, Meredith T. Robbins, Ronald Dubner, Ke Ren

Research output: Contribution to journalArticlepeer-review

Abstract

To study the glutamatergic mechanisms underlying changes in excitability in the brain stem pain modulatory circuitry after injury, we examined GluR1 serine 831 phosphorylation in the rostral ventromedial medulla (RVM) after complete Freund's adjuvant-induced hindpaw inflammation. Western blots indicated a rapid and prolonged (30 min and 7 days post-inflammation) increase in phosphoserine 831 GluR1 protein levels in the RVM. The upregulated GluR1 phosphorylation was blocked by pretreatment, but not by post-treatment, with the local anesthetic, lidocaine, at the site of inflammation. The upregulation of phosphoserine 831 GluR1 was attenuated by pretreatment with chelerythrine, a selective PKC inhibitor, KN-93, a selective CaMKII inhibitor, and two NMDA receptor antagonists, MK-801 and APV. These findings provide new evidence linking in vivo AMPA receptor phosphorylation in the RVM pain modulatory circuitry to the enhanced descending pain modulation after inflammation.

Original languageEnglish (US)
Pages (from-to)201-205
Number of pages5
JournalNeuroscience Letters
Volume366
Issue number2
DOIs
StatePublished - Aug 12 2004
Externally publishedYes

Keywords

  • AMPA
  • Descending modulation
  • GluR1
  • Inflammatory hyperalgesia
  • Phosphoserine 831 GluR1
  • Rostral ventromedial medulla

ASJC Scopus subject areas

  • Neuroscience(all)

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