TY - JOUR
T1 - Desafíos para la recolección de sangre y el análisis bioquímico en un gran estudio multicéntrico con adolescentes en las escuelas
T2 - Lecciones del ERICA en Brasil
AU - Cureau, Felipe Vogt
AU - Bloch, Katia Vergetti
AU - Henz, Aline
AU - Schaan, Camila W.
AU - Klein, Carlos Henrique
AU - De Oliveira, Cecília Lacroix
AU - Giannini, Denise Tavares
AU - De Leon, Elisa Brosina
AU - Abreu, Gabriela de Azevedo
AU - Telo, Gabriela Heiden
AU - Dias, Glauber Monteiro
AU - De Carvalho, Kênia Mara Baiocchi
AU - Barufaldi, Laura Augusta
AU - Kuschnir, Maria Cristina Caetano
AU - Szklo, Moyses
AU - Montenegro, Renan
AU - Da Silva, Thiago Luiz Nogueira
AU - Ekelund, Ulf
AU - Schaan, Beatriz D.
N1 - Funding Information:
ERICA was supported by Science & Technology Department/Science, Technology and Strategic Inputs Secretariat/Brazilian Ministry of Health (Decit/SCTIE/MS), Health Fund (CT-Saúde)/Brazilian Ministry of Science, Technology, Innovation and Communication (MCTI) by FINEP (grant: 01090421); CNPq (grants: 565037/2010-2, 405009/2012-7 and 457050/2013-6), and Research Incentive Fund, Hospital of Clinics of Porto Alegre (FIPE-HCPA; register: 09-098).
Publisher Copyright:
© 2017, Fundacao Oswaldo Cruz. All rights reserved.
PY - 2017
Y1 - 2017
N2 - The Study of Cardiovascular Risk in Adolescents (ERICA) is a pioneering study that aimed to assess the prevalence of cardiovascular risk factors, including metabolic syndrome components in Brazilian adolescents. This study aims to describe the methodological aspects related to blood collection as well as to report pertaining results of the preparation, transport, storage, and exams in ERICA. Exams in ERICA were performed in a single laboratory and blood samples were collected in schools in a standardized manner. Logistics involved air transportation of samples to the reference laboratory with controlled temperature since sample collection. The serum was stored in local biorepositories in four centers to be used in future analyses. During the study, 284,247 exams were performed and rate of participation in exams was 56.2%, thus involving 40,732 adolescents. From the total, 92.6% of the samples reached the reference laboratory maintaining the temperature between 0-10°C. No clinical significant changes in results due to temperature changes were identified. External quality control recorded satisfactory results in 98.7% of the evaluations. Four biorepositories with samples of 7,785 adolescents were created. Thus, we can consider that the logistics adopted in ERICA was fairly successful and description of this as well as the difficulties experienced in Brazil can inform and facilitate the planning of future studies, especially in developing countries.
AB - The Study of Cardiovascular Risk in Adolescents (ERICA) is a pioneering study that aimed to assess the prevalence of cardiovascular risk factors, including metabolic syndrome components in Brazilian adolescents. This study aims to describe the methodological aspects related to blood collection as well as to report pertaining results of the preparation, transport, storage, and exams in ERICA. Exams in ERICA were performed in a single laboratory and blood samples were collected in schools in a standardized manner. Logistics involved air transportation of samples to the reference laboratory with controlled temperature since sample collection. The serum was stored in local biorepositories in four centers to be used in future analyses. During the study, 284,247 exams were performed and rate of participation in exams was 56.2%, thus involving 40,732 adolescents. From the total, 92.6% of the samples reached the reference laboratory maintaining the temperature between 0-10°C. No clinical significant changes in results due to temperature changes were identified. External quality control recorded satisfactory results in 98.7% of the evaluations. Four biorepositories with samples of 7,785 adolescents were created. Thus, we can consider that the logistics adopted in ERICA was fairly successful and description of this as well as the difficulties experienced in Brazil can inform and facilitate the planning of future studies, especially in developing countries.
KW - Adolescent
KW - Biological specimen banks
KW - Blood specimen collection
KW - Cardiovascular diseases
KW - Clinical laboratory techniques
UR - http://www.scopus.com/inward/record.url?scp=85019934675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019934675&partnerID=8YFLogxK
U2 - 10.1590/0102-311X00122816
DO - 10.1590/0102-311X00122816
M3 - Article
C2 - 28538790
AN - SCOPUS:85019934675
SN - 0102-311X
VL - 33
JO - Cadernos de saude publica
JF - Cadernos de saude publica
IS - 4
M1 - e00122816
ER -