TY - JOUR
T1 - Chagas disease in Southern Coastal Ecuador
T2 - Coinfections with arboviruses and a comparison of serological assays for chagas disease diagnosis
AU - Mita-Mendoza, Neida K.
AU - McMahon, Elizabeth
AU - Kenneson, Aileen
AU - Barbachano-Guerrero, Arturo
AU - Beltran-Ayala, Efrain
AU - Cueva, Cinthya
AU - King, Christine A.
AU - Lupone, Christina D.
AU - Castro-Sesquen, Yagahira E.
AU - Gilman, Robert H.
AU - Endy, Timothy P.
AU - Stewart-Ibarra, Anna M.
N1 - Funding Information:
Acknowledgments: This study was supported in part by the Department of Defense Global Emerging Infection Surveillance (GEIS) grant (P0220_13_OT) and the Department of Medicine of SUNY Upstate Medical University. A. M. S. was additionally supported by NSF DEB EEID 1518681 and NSF DEB RAPID 1641145; E. M. by a Syracuse University Renee Crown Honors Program Crown Award; and A. B.-G. by a fellowship from the Mexican Council for Science and Technology (CONACYT). Many thanks to Ortho Clinical Diagnostics for the donation of the ORTHO T. cruzi ELISA test system kits.
Funding Information:
Financial support: Y. C. S. reports grants and nonfinancial support from Inbios International, other from Hemagen, during the conduct of the study; and grants and nonfinancial support from Inbios International outside the submitted work. E. M. reports grants from Syracuse University, during the conduct of the study.
PY - 2018
Y1 - 2018
N2 - Occurrence of Chagas disease and arbovirus coinfections is unknown, despite the vast co-endemic areas throughout the Americas. This study examined the proportion of individuals positive for Trypanosoma cruzi and coinfections with dengue, chikungunya, and Zika viruses in Machala, Ecuador (January 2014–December 2015). Chagas seropositivity was evaluated with five commercially available assays. Dengue infections were identified by nonstructural protein 1 rapid test and enzyme linked immunosorbent assay (ELISA), immunoglobulin M ELISA, and reverse transcription PCR (RT-PCR); chikungunya and Zika infections were identified by RT-PCR. Of 658 individuals, six were positive for T. cruzi (0.91%), including one T. cruzi/dengue coinfection and one T. cruzi/chikungunya/dengue coinfection. The clinical manifestations of coinfected individuals corresponded to severe dengue and dengue with warning signs, respectively. We observed discrepant results by using the Hemagen Chagas kit and the rapid test Chagas Detect Plus (false positives: 3.9% and 15.4%), highlighting the need to assess diagnostic assays in geographic regions with distinct taxonomic units of T. cruzi.
AB - Occurrence of Chagas disease and arbovirus coinfections is unknown, despite the vast co-endemic areas throughout the Americas. This study examined the proportion of individuals positive for Trypanosoma cruzi and coinfections with dengue, chikungunya, and Zika viruses in Machala, Ecuador (January 2014–December 2015). Chagas seropositivity was evaluated with five commercially available assays. Dengue infections were identified by nonstructural protein 1 rapid test and enzyme linked immunosorbent assay (ELISA), immunoglobulin M ELISA, and reverse transcription PCR (RT-PCR); chikungunya and Zika infections were identified by RT-PCR. Of 658 individuals, six were positive for T. cruzi (0.91%), including one T. cruzi/dengue coinfection and one T. cruzi/chikungunya/dengue coinfection. The clinical manifestations of coinfected individuals corresponded to severe dengue and dengue with warning signs, respectively. We observed discrepant results by using the Hemagen Chagas kit and the rapid test Chagas Detect Plus (false positives: 3.9% and 15.4%), highlighting the need to assess diagnostic assays in geographic regions with distinct taxonomic units of T. cruzi.
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U2 - 10.4269/ajtmh.18-0441
DO - 10.4269/ajtmh.18-0441
M3 - Article
C2 - 30350764
AN - SCOPUS:85058164698
VL - 99
SP - 1530
EP - 1533
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 6
ER -