Chagas disease in Southern Coastal Ecuador: Coinfections with arboviruses and a comparison of serological assays for chagas disease diagnosis

Neida K. Mita-Mendoza, Elizabeth McMahon, Aileen Kenneson, Arturo Barbachano-Guerrero, Efrain Beltran-Ayala, Cinthya Cueva, Christine A. King, Christina D. Lupone, Yagahira E. Castro-Sesquen, Robert H. Gilman, Timothy P. Endy, Anna M. Stewart-Ibarra

Research output: Contribution to journalArticlepeer-review

Abstract

Occurrence of Chagas disease and arbovirus coinfections is unknown, despite the vast co-endemic areas throughout the Americas. This study examined the proportion of individuals positive for Trypanosoma cruzi and coinfections with dengue, chikungunya, and Zika viruses in Machala, Ecuador (January 2014–December 2015). Chagas seropositivity was evaluated with five commercially available assays. Dengue infections were identified by nonstructural protein 1 rapid test and enzyme linked immunosorbent assay (ELISA), immunoglobulin M ELISA, and reverse transcription PCR (RT-PCR); chikungunya and Zika infections were identified by RT-PCR. Of 658 individuals, six were positive for T. cruzi (0.91%), including one T. cruzi/dengue coinfection and one T. cruzi/chikungunya/dengue coinfection. The clinical manifestations of coinfected individuals corresponded to severe dengue and dengue with warning signs, respectively. We observed discrepant results by using the Hemagen Chagas kit and the rapid test Chagas Detect Plus (false positives: 3.9% and 15.4%), highlighting the need to assess diagnostic assays in geographic regions with distinct taxonomic units of T. cruzi.

Original languageEnglish (US)
Pages (from-to)1530-1533
Number of pages4
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume99
Issue number6
DOIs
StatePublished - 2018

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Chagas disease in Southern Coastal Ecuador: Coinfections with arboviruses and a comparison of serological assays for chagas disease diagnosis'. Together they form a unique fingerprint.

Cite this