Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21

T. D. Mashkova, I. G. Tyumeneva, O. L. Zinov'eva, L. Yu Romanova, E. Jabs, I. A. Aleksandrov

Research output: Contribution to journalArticle

Abstract

A clone covering the euchromatin/heterochromatin boundary was isolated from a cosmid library specific for the human chromosome 21. This DNA contained a long block of the centromeric α-satellite DNA, unique nucleotide sequences, and dispersed Alu and L1 repeats. The α-satellite block constituted about a half of total length (about 45 kb) of the cloned DNA. It was adjacent to, but not overlapping with the nonsatellite region. The border between alphoid and nonalphoid domains was found in the longest subclone of 11 kb. This subclone was completely sequenced, and the sequence analyzed. The primary structure of an alphoid DNA of about 12 kb was shown to be different from the known chromosome 21-specific alphoid families and from the other described sequences. It was attributed to the suprachromosomal family 4 characterized by monomeric organization of the ancestral repeat. This alphoid DNA had no higher-order repeated structures typical of satellites. Insertions of one Alu repeat and three truncated L1 repeats were detected. One of the latter was flanked by direct repeats, as common for the mobile elements of the human genome.

Original languageEnglish (US)
Pages (from-to)617-625
Number of pages9
JournalMolecular Biology
Volume30
Issue number5 SUPPL. 1
StatePublished - Sep 1996
Externally publishedYes

Fingerprint

Euchromatin
Satellite DNA
Chromosomes, Human, Pair 21
Heterochromatin
Human Chromosomes
DNA
Cosmids
Nucleic Acid Repetitive Sequences
Human Genome
Clone Cells

Keywords

  • α-satellite DNA
  • Chromosome 21
  • Dispersed repeats
  • Euchromatin
  • Heterochromatin

ASJC Scopus subject areas

  • Genetics

Cite this

Mashkova, T. D., Tyumeneva, I. G., Zinov'eva, O. L., Romanova, L. Y., Jabs, E., & Aleksandrov, I. A. (1996). Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21. Molecular Biology, 30(5 SUPPL. 1), 617-625.

Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21. / Mashkova, T. D.; Tyumeneva, I. G.; Zinov'eva, O. L.; Romanova, L. Yu; Jabs, E.; Aleksandrov, I. A.

In: Molecular Biology, Vol. 30, No. 5 SUPPL. 1, 09.1996, p. 617-625.

Research output: Contribution to journalArticle

Mashkova, TD, Tyumeneva, IG, Zinov'eva, OL, Romanova, LY, Jabs, E & Aleksandrov, IA 1996, 'Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21', Molecular Biology, vol. 30, no. 5 SUPPL. 1, pp. 617-625.
Mashkova TD, Tyumeneva IG, Zinov'eva OL, Romanova LY, Jabs E, Aleksandrov IA. Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21. Molecular Biology. 1996 Sep;30(5 SUPPL. 1):617-625.
Mashkova, T. D. ; Tyumeneva, I. G. ; Zinov'eva, O. L. ; Romanova, L. Yu ; Jabs, E. ; Aleksandrov, I. A. / Centromeric α-satellite DNA at euchromatin/heterochromatin boundary of human chromosome 21. In: Molecular Biology. 1996 ; Vol. 30, No. 5 SUPPL. 1. pp. 617-625.
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