Centrifugation-augmented solid-phase immunoassay (Caspia) for the rapid diagnosis of infectious diseases

Paolo G. Miotti, Raphael P. Viscidi, Joseph Eiden, Eric Cerny, Robert H. Yolken

Research output: Contribution to journalArticle

Abstract

We have devised centrifugation-augmented solid-phase immunoassays (CASPIAs) for the detection of microbial pathogens in human body fluids. The CASPIA format combines several features of latex agglutination and solid-phase immunoassay systems to produce an assay system that is inexpensive and simple to perform. We found that CASPIA systems for the detection of rotaviral, group A streptococcal, and Haemophilus influenzae type b antigens were significantly more sensitive than latex agglutination assays and at least as sensitive as enzyme immunoassay systems using analogous reagents. Because CASPIA reactions could either be read macroscopically, recorded with a standard office photocopying device, or quantitated by means of a microplate colorimeter, the assays were applicable under a wide range of clinical and laboratory conditions.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalJournal of Infectious Diseases
Volume154
Issue number2
DOIs
StatePublished - Aug 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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