Central role of cyclooxygenase in the response of canine peripheral airways to antigen

S. R. Kleeberger, J. Kolbe, N. F. Adkinson, S. P. Peters, E. W. Spannhake

Research output: Contribution to journalArticle

Abstract

We studied the effects of antigen aerosol challenge on the airways of the canine peripheral lung and examined the roles of cyclooxygenase products, histamine, and cholinergic activity in the responses. One-minute deliveries of 1:10,000 or 1:100,000 concentrations of Ascaris suum antigen aerosol through a wedged bronchoscope resulted in mean maximal increases in collateral system resistance (Rcs) of 415 and 177%, respectively, after 4-8 min. Repeated antigen challenge (1:100,000) resulted in significantly decreased responsiveness to antigen after the initial exposure (P < 0.005). Bronchoalveolar lavage fluid obtained from the isolated, challenged segment had a significant increase in mean (± SE) prostaglandin D2 (PGD2) concentration vs. control (222.0 ± 65.3 vs. 72.7 ± 19.5 pg/ml; P < 0.05); histamine concentrations were variable and not significantly different (4.1 ± 2.6 vs. 1.2 ± 0.2 ng/ml; P > 0.05). In nine experiments, cyclooxygenase inhibition significantly attenuated the antigen-induced increase in Rcs by 53.4% (P < 0.001), and the concentration of PGD2 in lavage fluid was reduced by 96.0% (P < 0.01). Blockade of histamine H1-receptors (n = 8) or cholinergic receptors (n = 7) did not significantly affect the airway response (P > 0.05). These data indicate that the canine peripheral lung responds in a dose-dependent manner to antigen aerosol challenge and exhibits characteristics of antigen tachyphylaxis. Results also suggest that cyclooxygenase products play a central role in the acute bronchoconstrictive response of the lung periphery.

Original languageEnglish (US)
Pages (from-to)1309-1315
Number of pages7
JournalJournal of applied physiology
Volume61
Issue number4
StatePublished - Dec 1 1986

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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