Cellular uptake and intracellular transport of viral and non-viral gene vectors in differentiated neurons affected in Parkinson's Disease

Research output: Contribution to conferencePaper

Abstract

The cellular uptake and intracellular transport of viral and non-viral gene vectors in differentiated neurons affected in parkinson's diseases was discussed. Flow cytometry and real time confocal particle tracking (CPT) was used to elucidate the contribution of cellular uptake, endosomal escape, and cytoplasmic transport to the overall gene delivery process. CPT was used to quantitatively compare the intracellular quantitatively compare the intracellular transport and trafficking of low efficiency non-viral vectors (PEI/DNA) to high efficiency viral vectors in fully differentiated primary cortical neurons. Most internalized PEI/DNA nanocomplexes and adenoviruses were found in the perinuclear region of rat cortical neurons after 1h post transfection.

Original languageEnglish (US)
Pages1085-1086
Number of pages2
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

ASJC Scopus subject areas

  • Engineering(all)

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