Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques

Alexander Spira, Preston A. Marx, Bruce K. Patterson, James Mahoney, Richard A. Koup, Steven M. Wolinsky, David D. Ho

Research output: Contribution to journalArticle

Abstract

We used the simian immunodeficiency virus (SIV)/rhesus macaque model to study events that underlie sexual transmission of human immunodeficiency virus type 1 (HIV-1). Four female rhesus macaques were inoculated intravaginally with SIVmac251, and then killed 2, 5, 7, and 9 d later. A technique that detected polymerase chain reaction amplified SIV in situ showed that the first cellular targets for SIV were in the lamina propria of the cervicovaginal mucosa, immediately subjacent to the epithelium. Phenotypic and localization studies demonstrated that many of the infected cells were likely to be dendritic cells. Within 2 d of inoculation, infected cells were identified in the paracortex and subcapsular sinus of the draining internal iliac lymph nodes. Subsequently, systemic dissemination of SIV was rapid, since culturable virus was detectable in the blood by day 5. From these results, we present a model for mucosal transmission of SIV and HIV-1.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalJournal of Experimental Medicine
Volume183
Issue number1
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Simian Immunodeficiency Virus
Virus Diseases
Macaca mulatta
HIV-1
Mucous Membrane
Dendritic Cells
Epithelium
Lymph Nodes
Viruses
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques. / Spira, Alexander; Marx, Preston A.; Patterson, Bruce K.; Mahoney, James; Koup, Richard A.; Wolinsky, Steven M.; Ho, David D.

In: Journal of Experimental Medicine, Vol. 183, No. 1, 01.01.1996, p. 215-225.

Research output: Contribution to journalArticle

Spira, Alexander ; Marx, Preston A. ; Patterson, Bruce K. ; Mahoney, James ; Koup, Richard A. ; Wolinsky, Steven M. ; Ho, David D. / Cellular targets of infection and route of viral dissemination after an intravaginal inoculation of simian immunodeficiency virus into rhesus macaques. In: Journal of Experimental Medicine. 1996 ; Vol. 183, No. 1. pp. 215-225.
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