Cellular Signaling Circuits Interfaced with Synthetic, Post-Translational, Negating Boolean Logic Devices

Shiva Razavi, Steven Su, Takanari Inoue

Research output: Contribution to journalArticle

Abstract

A negating functionality is fundamental to information processing of logic circuits within cells and computers. Aiming to adapt unutilized electronic concepts to the interrogation of signaling circuits in cells, we first took a bottom-up strategy whereby we created protein-based devices that perform negating Boolean logic operations such as NOT, NOR, NAND, and N-IMPLY. These devices function in living cells within a minute by precisely commanding the localization of an activator molecule among three subcellular spaces. We networked these synthetic gates to an endogenous signaling circuit and devised a physiological output. In search of logic functions in signal transduction, we next took a top-down approach and computationally screened 108 signaling pathways to identify commonalities and differences between these biological pathways and electronic circuits. This combination of synthetic and systems approaches will guide us in developing foundations for deconstruction of intricate cell signaling, as well as construction of biomolecular computers. (Figure Presented).

Original languageEnglish (US)
Pages (from-to)676-685
Number of pages10
JournalACS Synthetic Biology
Volume3
Issue number9
DOIs
StatePublished - Sep 19 2014

Keywords

  • Rac
  • chemically induced dimerization
  • membrane ruffling
  • negation
  • signaling pathway
  • synthetic Boolean logic

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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