TY - JOUR
T1 - Cellular resolution in clinical MALDI mass spectrometry imaging
T2 - The latest advancements and current challenges
AU - Ščupáková, Klára
AU - Balluff, Benjamin
AU - Tressler, Caitlin
AU - Adelaja, Tobi
AU - Heeren, Ron M.A.
AU - Glunde, Kristine
AU - Ertaylan, Gökhan
N1 - Publisher Copyright:
© 2019 Walter de Gruyter GmbH, Berlin/Boston 2019.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Mass spectrometry (MS) is the workhorse of metabolomics, proteomics and lipidomics. Mass spectrometry imaging (MSI), its extension to spatially resolved analysis of tissues, is a powerful tool for visualizing molecular information within the histological context of tissue. This review summarizes recent developments in MSI and highlights current challenges that remain to achieve molecular imaging at the cellular level of clinical specimens. We focus on matrix-assisted laser desorption/ionization (MALDI)-MSI. We discuss the current status of each of the analysis steps and remaining challenges to reach the desired level of cellular imaging. Currently, analyte delocalization and degradation, matrix crystal size, laser focus restrictions and detector sensitivity are factors that are limiting spatial resolution. New sample preparation devices and laser optic systems are being developed to push the boundaries of these limitations. Furthermore, we review the processing of cellular MSI data and images, and the systematic integration of these data in the light of available algorithms and databases. We discuss roadblocks in the data analysis pipeline and show how technology from other fields can be used to overcome these. Finally, we conclude with curative and community efforts that are needed to enable contextualization of the information obtained.
AB - Mass spectrometry (MS) is the workhorse of metabolomics, proteomics and lipidomics. Mass spectrometry imaging (MSI), its extension to spatially resolved analysis of tissues, is a powerful tool for visualizing molecular information within the histological context of tissue. This review summarizes recent developments in MSI and highlights current challenges that remain to achieve molecular imaging at the cellular level of clinical specimens. We focus on matrix-assisted laser desorption/ionization (MALDI)-MSI. We discuss the current status of each of the analysis steps and remaining challenges to reach the desired level of cellular imaging. Currently, analyte delocalization and degradation, matrix crystal size, laser focus restrictions and detector sensitivity are factors that are limiting spatial resolution. New sample preparation devices and laser optic systems are being developed to push the boundaries of these limitations. Furthermore, we review the processing of cellular MSI data and images, and the systematic integration of these data in the light of available algorithms and databases. We discuss roadblocks in the data analysis pipeline and show how technology from other fields can be used to overcome these. Finally, we conclude with curative and community efforts that are needed to enable contextualization of the information obtained.
KW - data analysis
KW - data integration and interpretation
KW - histopathology
KW - mass spectrometry imaging
KW - molecular analysis
KW - spatial resolution
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U2 - 10.1515/cclm-2019-0858
DO - 10.1515/cclm-2019-0858
M3 - Review article
C2 - 31665113
AN - SCOPUS:85074636443
VL - 58
SP - 914
EP - 929
JO - Zeitschrift fur klinische Chemie und klinische Biochemie
JF - Zeitschrift fur klinische Chemie und klinische Biochemie
SN - 1434-6621
IS - 6
ER -