This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.
|Original language||English (US)|
|Pages (from-to)||Unit 1.8|
|Journal||Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]|
|State||Published - Aug 2001|
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