Abstract
This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.
| Original language | English (US) |
|---|---|
| Pages (from-to) | Unit 1.8 |
| Journal | Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] |
| Volume | Chapter 1 |
| State | Published - Aug 2001 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Neuroscience