Cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH).

J. F. Guzowski, P. F. Worley

Research output: Contribution to journalArticlepeer-review

Abstract

This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.

Original languageEnglish (US)
Pages (from-to)Unit 1.8
JournalCurrent protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
VolumeChapter 1
StatePublished - Aug 2001
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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