@inbook{57c7581ab87b40d38d237e3581c9f80d,
title = "Cellular application of genetically encoded sensors and impeders of AMPK",
abstract = "Unraveling the spatiotemporal dynamics of 5'-AMP-activated protein kinase (AMPK) signaling is necessary to bridge the gap between nutrient signaling and downstream function. Three genetically encoded F{\"o}rster Resonance Energy Transfer (FRET)-based AMPK biosensors are available yielding insight into how AMPK-derived signal propagates throughout a cell in response to particular inputs. These findings, together with accumulating evidence obtained from biochemical techniques, promise to give a holistic understanding of the AMPK signaling. In this protocol, we describe the procedures and materials required for imaging intracellular AMPK activity in an organelle-specific manner, with a focus on ABKAR, a FRET-based biosensor. In addition, we introduce a novel AMPK inhibitor peptide that allows us to inhibit AMPK activity at specific subcellular compartments.",
keywords = "Biosensor, Compartmentalization, F{\"o}rster resonance energy transfer (FRET), Inhibitor peptide, Kinase activity monitoring, Organelle-specific, Signaling dynamics",
author = "Takafumi Miyamoto and Elmer Rho and Allen Kim and Takanari Inoue",
year = "2018",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-7598-3_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "255--272",
booktitle = "Methods in Molecular Biology",
}