TY - JOUR
T1 - Cellular and biophysical evidence for interactions between adenosine triphosphate and P-glycoprotein substrates
T2 - Functional implications for adenosine triphosphate/drug cotransport in P-glycoprotein overexpressing tumor cells and in P-glycoprotein low-level expressing erythrocytes
AU - Abraham, Edward H.
AU - Shrivastav, Brij
AU - Salikhova, Anna Y.
AU - Sterling, Kenneth M.
AU - Johnston, Nicholas
AU - Guidotti, Guido
AU - Scala, Stefania
AU - Litman, Thomas
AU - Chan, King C.
AU - Arceci, Robert J.
AU - Steiglitz, Kim
AU - Herscher, Laurie
AU - Okunieff, Paul
PY - 2001/1/1
Y1 - 2001/1/1
N2 - P-glycoprotein is involved with the removal of drugs, most of them cations, from the plasma membrane and cytoplasm. Pgp is also associated with movement of ATP, an anion, from the cytoplasm to the extracellular space. The central question of this study is whether drug and ATP transport associated with the expression of Pgp are in any way coupled. We have measured the stoichiometry of transport coupling between drug and ATP release. The drug and ATP transport that is inhibitable by the sulfonylurea compound, glyburide (P. E. Golstein, A. Boom, J. van Geffel, P. Jacobs, B. Masereel, and R. Beauwens, Pfluger's Arch. 437, 652, 1999), permits determination of the transport coupling ratio, which is close to 1:1. In view of this result, we asked whether ATP interacts directly with Pgp substrates. We show by measuring the movement of Pgp substrates in electric fields that ATP and drug movement are coupled. The results are compatible with the view that substrates for Pgp efflux are driven by the movement of ATP through electrostatic interaction and effective ATP-drug complex formation with net anionic character. This mechanism not only pertains to drug efflux from tumor cells overexpressing Pgp, but also provides a framework for understanding the role of erythrocytes in drug resistance. The erythrocyte consists of a membrane surrounding a millimolar pool of ATP. Mammalian RBCs have no nucleus or DNA drug/toxin targets. From the perspective of drug/ATP complex formation, the RBC serves as an important electrochemical sink for toxins. The presence in the erythrocyte membrane of approximately 100 Pgp copies per RBC provides a mechanism for eventual toxin clearance. The RBC transport of toxins permits their removal from sensitive structures and ultimate clearance from the organism via the liver and/or kidneys.
AB - P-glycoprotein is involved with the removal of drugs, most of them cations, from the plasma membrane and cytoplasm. Pgp is also associated with movement of ATP, an anion, from the cytoplasm to the extracellular space. The central question of this study is whether drug and ATP transport associated with the expression of Pgp are in any way coupled. We have measured the stoichiometry of transport coupling between drug and ATP release. The drug and ATP transport that is inhibitable by the sulfonylurea compound, glyburide (P. E. Golstein, A. Boom, J. van Geffel, P. Jacobs, B. Masereel, and R. Beauwens, Pfluger's Arch. 437, 652, 1999), permits determination of the transport coupling ratio, which is close to 1:1. In view of this result, we asked whether ATP interacts directly with Pgp substrates. We show by measuring the movement of Pgp substrates in electric fields that ATP and drug movement are coupled. The results are compatible with the view that substrates for Pgp efflux are driven by the movement of ATP through electrostatic interaction and effective ATP-drug complex formation with net anionic character. This mechanism not only pertains to drug efflux from tumor cells overexpressing Pgp, but also provides a framework for understanding the role of erythrocytes in drug resistance. The erythrocyte consists of a membrane surrounding a millimolar pool of ATP. Mammalian RBCs have no nucleus or DNA drug/toxin targets. From the perspective of drug/ATP complex formation, the RBC serves as an important electrochemical sink for toxins. The presence in the erythrocyte membrane of approximately 100 Pgp copies per RBC provides a mechanism for eventual toxin clearance. The RBC transport of toxins permits their removal from sensitive structures and ultimate clearance from the organism via the liver and/or kidneys.
KW - ABC proteins
KW - ATP (adenosine triphosphate)
KW - ATP transporter
KW - Adriamycin
KW - Apyrase
KW - CD39
KW - Camptothecin
KW - Capillary electrophoresis
KW - Colchicine
KW - Cotransport
KW - Doxorubicin
KW - Electrophoresis
KW - Erythrocyte
KW - Knockout mice
KW - MDR (multidrug resistance protein)
KW - Pgp (P-glycoprotein)
KW - Pgp drug substrates
KW - Plasma ATP
KW - RBC
KW - Stoichiometry of coupling
KW - Taxol
KW - Verapamil
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U2 - 10.1006/bcmd.2000.0373
DO - 10.1006/bcmd.2000.0373
M3 - Article
C2 - 11358379
AN - SCOPUS:0035054299
SN - 1079-9796
VL - 27
SP - 181
EP - 200
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
IS - 1
ER -