TY - JOUR
T1 - Cell‐specific constraints to the lateral diffusion of a membrane glycoprotein
AU - Barbour, Suzanne
AU - Edidin, Michael
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1992/3
Y1 - 1992/3
N2 - We have previously shown that the lateral diffusion, D, of the class I Major Histocompatibility Complex (MHC) glycoprotein H‐2Ld is constrained by its glycosylation, when expressed in mouse L‐cells. Removal of one or more of the 3 N‐linked olisaccharides of H‐2Ld glycoproteins results in an increase in D. In order to further examine the influence of glycosylation on D, we compared lateral diffusion of H‐2Ld expressed in wild‐type CHO cells with lateral diffusion of the same molecule expressed in mutant CHO cells with aberrant surface glycosylation. In addition, we compared lateral diffusion of wild‐type and unglycosylated H‐2Ld antigens in these cells. In contrast to the large effect of glycosylation state on lateral diffusion of H‐2Ld in mouse L‐cells. There was little effect of glycosylation on lateral diffusion of H‐2Ld in any of the CHO cells. This, together with similar results on hamster class I antigens, indicates that the constraints to D of H‐2Ld and other class I MHC molecules are different in CHO cells than in L‐cells. Measurements of lateral diffusion after treatment of cells with cytochalasin D make it clear that interactions between MHC class I molecules and a cytoskeleton are important in reducing the mobile fraction of diffusing molecules, R, though they cannot be shown to directly affect the diffusion coefficient, D.
AB - We have previously shown that the lateral diffusion, D, of the class I Major Histocompatibility Complex (MHC) glycoprotein H‐2Ld is constrained by its glycosylation, when expressed in mouse L‐cells. Removal of one or more of the 3 N‐linked olisaccharides of H‐2Ld glycoproteins results in an increase in D. In order to further examine the influence of glycosylation on D, we compared lateral diffusion of H‐2Ld expressed in wild‐type CHO cells with lateral diffusion of the same molecule expressed in mutant CHO cells with aberrant surface glycosylation. In addition, we compared lateral diffusion of wild‐type and unglycosylated H‐2Ld antigens in these cells. In contrast to the large effect of glycosylation state on lateral diffusion of H‐2Ld in mouse L‐cells. There was little effect of glycosylation on lateral diffusion of H‐2Ld in any of the CHO cells. This, together with similar results on hamster class I antigens, indicates that the constraints to D of H‐2Ld and other class I MHC molecules are different in CHO cells than in L‐cells. Measurements of lateral diffusion after treatment of cells with cytochalasin D make it clear that interactions between MHC class I molecules and a cytoskeleton are important in reducing the mobile fraction of diffusing molecules, R, though they cannot be shown to directly affect the diffusion coefficient, D.
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U2 - 10.1002/jcp.1041500313
DO - 10.1002/jcp.1041500313
M3 - Article
C2 - 1537882
AN - SCOPUS:0026604944
VL - 150
SP - 526
EP - 533
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 3
ER -