Cell viability and noninvasive in vivo MRI tracking of 3D cell encapsulating self-assembled microcontainers

Barjor Gimi, Dmitri Artemov, Timothy Leong, David H. Gracias, Wesley Gilson, Matthias Stuber, Zaver M. Bhujwalla

Research output: Contribution to journalArticlepeer-review

Abstract

Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of cell encapsulation. Negative contrast was obtained to discern the microcontainer with MRI; positive contrast was obtained in the complete absence of background signal. MRI on a clinical scanner highlights the translational nature of this research. The microcontainers were loaded with cells that were dispersed in an extracellular matrix, and implanted both subcutaneously and in human tumor xenografts in SCID mice. MRI was performed on the implants, and microcontainers retrieved postimplantation showed cell viability both within and proximal to the implant. The microcontainers are characterized by their small size, three dimensionality, controlled porosity, ease of parallel fabrication, chemical and mechanical stability, and noninvasive traceability in vivo.

Original languageEnglish (US)
Pages (from-to)403-408
Number of pages6
JournalCell Transplantation
Volume16
Issue number4
DOIs
StatePublished - 2007

Keywords

  • Cell encapsulation
  • Cell therapy
  • Implantation
  • Magnetic resonance microscopy
  • Microcapsules
  • Self-assembly

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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